ArticlePoultry science2026
Effects of protease-hydrolyzed cottonseed protein on growth performance, intestinal morphology, and bacterial diversity in broilers.
Article in Poultry science, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
1 citing paper in PubMed.
- Effects of Single-Cell Protein Derived fromAnimals : an open access journal from MDPI · 2026Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The present study aimed to investigate the effects of enzymatically hydrolyzed cottonseed protein (EHCP) on growth performance, intestinal morphology, and bacterial diversity of broilers. A total of 480 one-day-old yellow-feathered broilers were randomly allocated into 5 groups with 8 replicates per group and 12 broilers per replicate, and the experimental period lasted for 63 days. The control group was fed a corn-soybean meal based basal diet. In the experimental groups, dietary protein from corn-soybean meal was partially replaced with EHCP at replacement levels of 25 %, 50 %, 75 %, and 100 %. During the period of 1-63 days of age, no significant differences were observed in body weight (BW), average daily gain (ADG), or feed conversion ratio (FCR) among all groups (P > 0.05). Compared with the control group, the average daily feed intake (ADFI) in the 50 %, 75 %, and 100 % substitution groups was significantly decreased (P < 0.05). At 63 days of age, compared with the control group, the digestibility of dry matter (DM), ether extract (EE), and organic matter (OM) was significantly decreased in the 25 %, 50 %, 75 %, and 100 % substitution groups; the crude protein (CP) digestibility in the 100 % substitution group was significantly lower than that in all other treatment groups; and the gross energy (GE) digestibility in the 50 % and 100 % substitution groups was significantly lower than that in the control group (P < 0.05). No significant differences were observed in villus height, crypt depth, and villus height/crypt depth ratio (V/C ratio) in the duodenum, jejunum, and ileum among all groups (P > 0.05). 16S rDNA sequencing of cecal bacteria showed that compared with the control group, the Chao1, faith_pd, and observed-features indices in the 50 %, 75 %, and 100 % substitution groups were significantly increased, and the Simpson index in the 25 % and 75 % substitution groups was significantly higher than that in the 100 % substitution group (P < 0.05). Correlation analysis between differential cecal microbiota and volatile fatty acids (VFA) revealed that Mediterraneibacter was positively correlated with VFA, with a significant difference in relation to isobutyric acid, while Borkfalkia was negatively correlated with VFA, with a significant difference in relation to propionic acid (P < 0.05). In conclusion, the partial replacement of SBM with EHCP at an appropriate ratio in broiler diets had no adverse effects on broiler growth performance, intestinal morphology, or gut bacterial diversity. Considering the comprehensive indices of growth performance, nutrient metabolism and cecal microbial diversity, this study suggests that the maximum replacement level of EHCP should not exceed 75 %.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.