Evidence map›Paper›PMID 41933352›Full record

ArticleBiotechnology for biofuels and bioproducts2026

Metabolic engineering of Bacillus subtilis for high-yield surfactin production.

Yong Wang, Hanqing Li, Yue Mao, Pengfei Li, Haizhen Wu, Jiang Ye, Huizhan Zhang

Abstract read
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In one paragraph

Article in Biotechnology for biofuels and bioproducts, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Yong WangState Key Laboratory of Bioreactor Engineering, Department of Applied Biology, School of Biotechnology, East China University of Science and Technology, 130 Meilong Road, Shanghai, 200237, People's Republic of China.
Hanqing LiState Key Laboratory of Bioreactor Engineering, Department of Applied Biology, School of Biotechnology, East China University of Science and Technology, 130 Meilong Road, Shanghai, 200237, People's Republic of China.
Yue MaoState Key Laboratory of Bioreactor Engineering, Department of Applied Biology, School of Biotechnology, East China University of Science and Technology, 130 Meilong Road, Shanghai, 200237, People's Republic of China.
Pengfei LiState Key Laboratory of Bioreactor Engineering, Department of Applied Biology, School of Biotechnology, East China University of Science and Technology, 130 Meilong Road, Shanghai, 200237, People's Republic of China.
Haizhen WuState Key Laboratory of Bioreactor Engineering, Department of Applied Biology, School of Biotechnology, East China University of Science and Technology, 130 Meilong Road, Shanghai, 200237, People's Republic of China.
Jiang YeState Key Laboratory of Bioreactor Engineering, Department of Applied Biology, School of Biotechnology, East China University of Science and Technology, 130 Meilong Road, Shanghai, 200237, People's Republic of China. yyjj413@163.com.
Huizhan ZhangState Key Laboratory of Bioreactor Engineering, Department of Applied Biology, School of Biotechnology, East China University of Science and Technology, 130 Meilong Road, Shanghai, 200237, People's Republic of China. huizhzh@ecust.edu.cn.

Funding

National Natural Science Foundation of China 41530318
6 · The paper itself

Abstract

The industrial application of surfactin has been constrained by the low production capacity of Bacillus subtilis. This study developed a high-yield, high-tolerance surfactin-producing strain through a multi-level metabolic engineering strategy. First, we constructed an enhanced P43 promoter (P43LN) by extending its upstream region from 260 to 520 bp and pairing it with a cognate 5' UTR, which exhibited more than threefold higher activity than the commonly used core P43 promoter, providing a versatile tool for stable and high-level expression of key genes. Based on this, the native srfA promoter in the oilfield-derived B. subtilis TD7 was replaced with the P43 promoter, yielding the engineered strain TP1. Subsequently, systematic knockout of competing antimicrobial peptide synthesis gene clusters (pps, bac, and pks) increased surfactin titer to 2.78 g/L. Further synergistic enhancement of NADPH regeneration (via zwf overexpression) and cellular tolerance (via efflux pump gene yerP overexpression) resulted in the final strain TS8, which achieved a shake-flask surfactin titer of 3.51 g/L, representing a 102.7% increase in per-OD

Indexed as

Bacillus subtilisMetabolic engineeringP43 promoterSurfactinTolerance engineering

Identifiers

PMID41933352
PMCPMC13173703

What OpenQuestion holds

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.