Evidence map›Paper›PMID 41932338›Full record

ArticleStem cell reports2026

A semi-automated MEA spike sorting method for high-throughput assessment of cultured neurons.

Xiaoxuan Ren, Carissa L Sirois, Raymond Doudlah, Ethan E Dayley, Natasha M Méndez-Albelo, Aviad Hai, Ari Rosenberg, Xinyu Zhao

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Stem cell reports, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

8 authors.

Xiaoxuan RenDepartment of Biomedical Engineering, University of Wisconsin-Madison, Madison, WI 53705, USA; Department of Electrical and Computer Engineering, University of Wisconsin - Madison, Madison, WI 53706, USA.
Carissa L SiroisWaisman Center, University of Wisconsin-Madison, Madison, WI 53705, USA; Department of Neuroscience, School of Medicine and Public Health, University of Wisconsin-Madison, Madison, WI 53705, USA.
Raymond DoudlahDepartment of Neuroscience, School of Medicine and Public Health, University of Wisconsin-Madison, Madison, WI 53705, USA.
Ethan E DayleyWaisman Center, University of Wisconsin-Madison, Madison, WI 53705, USA; Department of Neuroscience, School of Medicine and Public Health, University of Wisconsin-Madison, Madison, WI 53705, USA.
Natasha M Méndez-AlbeloWaisman Center, University of Wisconsin-Madison, Madison, WI 53705, USA; Department of Neuroscience, School of Medicine and Public Health, University of Wisconsin-Madison, Madison, WI 53705, USA; Molecular Cellular Pharmacology Training Program, University of Wisconsin-Madison, Madison, WI 53705, USA.
Aviad HaiDepartment of Biomedical Engineering, University of Wisconsin-Madison, Madison, WI 53705, USA; Department of Electrical and Computer Engineering, University of Wisconsin - Madison, Madison, WI 53706, USA. Electronic address: ahai@wisc.edu.
Ari RosenbergDepartment of Neuroscience, School of Medicine and Public Health, University of Wisconsin-Madison, Madison, WI 53705, USA. Electronic address: ari.rosenberg@wisc.edu.
Xinyu ZhaoWaisman Center, University of Wisconsin-Madison, Madison, WI 53705, USA; Department of Neuroscience, School of Medicine and Public Health, University of Wisconsin-Madison, Madison, WI 53705, USA. Electronic address: xinyu.zhao@wisc.edu.

Funding

Waisman Center Intellectual and Developmental Disabilities Research CenterP50HD105353 · NICHD · UNIVERSITY OF WISCONSIN-MADISON · PI Brittany Gail Travers · 2021 to 2026
$8.5M
Hierarchical cortical circuits implementing robust 3D visual perceptionR01EY029438 · NEI · UNIVERSITY OF WISCONSIN-MADISON · PI Ari Rosenberg · 2018 to 2026
$3.8M
Graduate Training in Molecular and Cellular PharmacologyT32GM141013 · NIGMS · UNIVERSITY OF WISCONSIN-MADISON · PI Anjon Audhya, Aaron Matthew LeBeau · 2021 to 2026
$3.2M
The role of RNA binding protein in FXR1P in interneuronsR01MH118827 · NIMH · UNIVERSITY OF WISCONSIN-MADISON · PI ZHAO, XINYU · 2019 to 2023
$2.2M
Interrogate FMRP functions in primate brain developmentR01MH136152 · NIMH · UNIVERSITY OF WISCONSIN-MADISON · PI Xinyu Zhao · 2024 to 2026
$2.2M
FMRP regulation of mitochondria and metabolism in mammalian brain developmentR01NS138268 · NINDS · UNIVERSITY OF WISCONSIN-MADISON · PI Xinyu Zhao · 2024 to 2026
$1.9M
NEI NIH HHS R01 EY029438NICHD NIH HHS P50 HD105353NIGMS NIH HHS T32 GM141013NIMH NIH HHS R01 MH118827NIMH NIH HHS R01 MH136152NINDS NIH HHS R01 NS138268
6 · The paper itself

Abstract

Neurons derived from human pluripotent stem cells (hPSCs) are valuable models for studying brain development and developing therapies for brain disorders. Evaluating hPSC-derived neurons requires assessing their electrical activity, which can be achieved using multi-electrode arrays (MEAs) for extracellular recordings. Because each electrode channel generally detects activity from multiple neurons, resolving the activity of single neurons requires a process called spike sorting. However, currently available methods were not developed for analyzing data from hPSC-derived neurons and require complex workflows and time-consuming manual intervention. Here, we introduce a semi-automated MEA spike sorting software (SAMS) designed specifically for low-density MEA recordings of cultured neurons. SAMS outperforms commercially available automated spike sorting algorithms in terms of accuracy and greatly reduces computational and human processing time. By providing an accessible, efficient, and integrated platform for spike sorting, SAMS enhances the resolution and utility of MEA in disease modeling and drug development using hPSC-derived neurons.

Indexed as

Action PotentialsHigh-Throughput Screening AssaysNeuronsAlgorithmsCells, CulturedHumansPluripotent Stem CellsSoftwareeletrophysiologicalhuman iPSCsmulti-electrode arrayneuronal activityneuronsopen sourcepluripotent stem cellssemi-automatedspike sortinguser-friendly

Identifiers

PMID41932338
PMCPMC13083792

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.