Protocol for immunomagnetic enrichment of CD8 T cells from complex murine tissues.

Ella Trolio, Lelinh Duong, Michaela Lucas, Amy Prosser

Abstract read

In one paragraph

Article in STAR protocols, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it

0cells of the map it votes in

0citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Ella TrolioMedical School, University of Western Australia, Perth, WA 6009, Australia. Electronic address: ella.trolio@uwa.edu.au.

Lelinh DuongMedical School, University of Western Australia, Perth, WA 6009, Australia.

Michaela LucasMedical School, University of Western Australia, Perth, WA 6009, Australia; Department of Immunology, Sir Charles Gairdner Hospital and PathWest Laboratory Medicine, Perth, WA 6009, Australia.

Amy ProsserMedical School, University of Western Australia, Perth, WA 6009, Australia. Electronic address: amy.prosser@uwa.edu.au.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Efficient isolation of CD8 T cells is essential for accurately investigating their roles in immune regulation and disease pathogenesis. Here, we present a protocol for the isolation and enrichment of CD8 T cells by positive selection from complex murine tissues, including bone marrow (BM), liver, heart, and kidneys. We describe steps for tissue harvesting and processing into single-cell suspensions. We then detail procedures for immunomagnetic CD8 T cell enrichment and assessment of cell purity and viability by flow cytometry.

Indexed as

CD8-Positive T-LymphocytesImmunomagnetic SeparationAnimalsCell SeparationFlow CytometryKidneyLiverMiceMyocardiumCell isolationCell separation/fractionationFlow CytometryImmunologyModel Organisms

Identifiers

PMID41931394

DOI10.1016/j.xpro.2026.104456

PMCPMC13084401

What OpenQuestion holds

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LicenceCC BY

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.