ArticleFrontiers in oncology2026
Local Promoter Methylation Disorder algorithm reveals bidirectional epigenetic disruption in DNMT3A-mutated AML and predicts azacitidine treatment response.
Article in Frontiers in oncology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
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1 citing paper in PubMed.
- Unrevealing the Epigenetic Landscape: SOX-2 and OCT-4 methylation in acute myeloid leukemia and myelodysplastic neoplasm.Discoveries (Craiova, Romania)Article
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4 authors.
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Abstract
Background: DNMT3A mutations occur in 20-25% of acute myeloid leukemia (AML) cases and are associated with poor prognosis, yet the epigenetic mechanisms underlying treatment response remain poorly understood. Traditional methylation analyses focus on mean levels, overlooking the heterogeneity that may be central to therapeutic vulnerability. We developed a Local Promoter Methylation Disorder (LPMD) algorithm to quantify methylation instability and evaluate its clinical utility in predicting azacitidine response. Methods: The LPMD algorithm was developed using the GSE62298 dataset (68 AML patients: 15 DNMT3A-mutant, 53 wild-type) to quantify local methylation heterogeneity through a 1-kb sliding window across CpG sites. Algorithm performance was validated in an independent WGBS cohort (20 AML samples) and further confirmed via R882-stratified analysis in the TCGA-LAML cohort (n = 194). Clinical predictive value was assessed in GSE152710 (63 high-risk MDS/secondary AML patients receiving azacitidine), where differentially methylated disorder regions (DMDRs) were identified and a consensus feature selection strategy was employed to construct a predictive panel. Longitudinal samples (n = 153) enabled treatment dynamics analysis. Results: The LPMD algorithm effectively captured DNMT3A mutation-associated epigenetic instability (Cohen's d = 0.8, Conclusions: The LPMD algorithm reframes DNMT3A-mutant AML from a hypomethylation paradigm to a methylation disorder paradigm, revealing dual mechanisms of aberrant stabilization and maintenance failure at distinct genomic regions. The 5-DMDR panel offers a practical tool for azacitidine response prediction, while dynamic LPMD monitoring provides a potential biomarker for therapeutic guidance. These findings establish methylation disorder as a clinically actionable dimension of epigenetic dysregulation in myeloid malignancies.
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