ArticleFrontiers in veterinary science2026
Flow cytometric evaluation of monoclonal antibodies for cross-reactivity with feline leukocytes.
Article in Frontiers in veterinary science, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
1 citing paper in PubMed.
- Body condition, age, and sex as modulators of peripheral blood immune-cell populations and lymphocyte proliferation in cats.Frontiers in veterinary science · 2026Article
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4 authors.
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No grant is acknowledged in the PubMed record.
Abstract
Monoclonal antibodies are essential tools for immunophenotyping, yet their use in feline flow cytometry is highly restricted due to the scarcity of cat-specific antibodies. Moreover, data on cross-reactivity with feline peripheral blood are limited. In this study, peripheral blood from four healthy cats was analysed using flow cytometry to assess cross-reactivity of 72 monoclonal antibodies, including 55 CD markers, 4 chemokine receptors, 7 cytokines, and 6 miscellaneous markers. Cross-reactivity was observed for 35 antibodies. Temporal effects on signal detection were evaluated using a long-term stability assessment of a staining panel comprising CD4, CD5, CD8, CD21, CD56, CD80, and Ki67. Signal detection was optimal immediately after staining and declined over time, and inter-individual variability among the cats was noted. These findings provide a validated set of cross-reactive antibodies and highlight temporal effects on signal detection in peripheral feline blood, supporting reliable flow cytometric analyses.
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