Layered Single-Cell Heterogeneity in Hormone Receptor Signaling Across Mouse Organoids and Human ERα+ Cancer Cells.

Pelin Yasar, Christopher R Day, Brian D Bennett, Jackson A Hoffman, Laura G Kammel, Trevor K Archer, Joseph Rodriguez

Abstract readPreprint

In one paragraph

Article in bioRxiv : the preprint server for biology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it

0cells of the map it votes in

0citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Pelin YasarSingle Cell Dynamics Group, Epigenetics and RNA Biology Laboratory, National Institute of Environmental Health Sciences, Research Triangle Park, Durham, NC 27709, USA.

Christopher R DaySingle Cell Dynamics Group, Epigenetics and RNA Biology Laboratory, National Institute of Environmental Health Sciences, Research Triangle Park, Durham, NC 27709, USA.

Brian D BennettIntegrative Bioinformatics Support Group, Biostatistics and Computational Biology Branch, National Institute of Environmental Health Sciences, Research Triangle Park, Durham, NC 27709, USA.

Jackson A HoffmanChromatin & Gene Expression Group, Epigenetics and RNA Biology Laboratory, National Institute of Environmental Health Sciences, Research Triangle Park, Durham, NC 27709, USA.

Laura G KammelSingle Cell Dynamics Group, Epigenetics and RNA Biology Laboratory, National Institute of Environmental Health Sciences, Research Triangle Park, Durham, NC 27709, USA.

Trevor K ArcherChromatin & Gene Expression Group, Epigenetics and RNA Biology Laboratory, National Institute of Environmental Health Sciences, Research Triangle Park, Durham, NC 27709, USA.ORCID 0000-0001-7651-3644

Joseph RodriguezSingle Cell Dynamics Group, Epigenetics and RNA Biology Laboratory, National Institute of Environmental Health Sciences, Research Triangle Park, Durham, NC 27709, USA.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Hormone receptor signaling is often interpreted through receptor abundance as a proxy for hormone responsiveness, yet the determinants of single-cell variability in hormone response remain unclear. Using murine mammary organoids, we mapped estrogen (E2) and progesterone (P4) responses at single-cell resolution. Despite controlled 3D culture conditions, basal cell-derived organoids exhibit striking variability in hormone-induced transcriptional responses, with ERα

Identifiers

PMID41929145

DOI10.64898/2026.03.19.712456

PMCPMC13041914

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.