ArticleResearch square2026
Macromolecular crowding alters transcription: real-time measurements with SYBR Green II.
Article in Research square, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Authors and funding
5 authors.
Funding
Abstract
Various factors affect transcription, including regulatory proteins, promoter sequences, ionic conditions, and nucleotide concentrations. Gel electrophoresis of radiolabeled RNA is a widely used assay to quantify transcript production in vitro. However, the use of radioactive reagents requires hazardous chemical training as well as specialized protocols and imaging systems. Moreover, dynamic monitoring of in vitro transcription could provide insight into conditions affecting the reaction. Widely available microplate readers and quantitative PCR instruments could be broadly adopted to study the kinetics and yields of transcription reactions with a suitable fluorescence assay. The SYBR Green II fluorophore has high affinity for RNA and can be used to monitor RNA production from
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Registered trials
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