Evidence map›Paper›PMID 41928506›Full record

ArticleStructure (London, England : 1993)2026

Cryo-ET reveals distinct Gag lattice architectures in virus-like particles and immature HIV-1.

Benjamin Preece, Wiley Peppel, Haley Durden, Rodrigo Gallegos, Gabriel Clinger, Nicole Bohn, Antje Huwendiek-Poser, Gillian Ysassi, Allyson Roman, Giovanna Garcia and 3 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Structure (London, England : 1993), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

13 authors.

Benjamin PreeceCenter for Cell and Genome Science, University of Utah, Salt Lake City, UT, USA; Department of Physics and Astronomy, University of Utah, Salt Lake City, UT, USA.
Wiley PeppelCenter for Cell and Genome Science, University of Utah, Salt Lake City, UT, USA; Department of Physics and Astronomy, University of Utah, Salt Lake City, UT, USA.
Haley DurdenCenter for Cell and Genome Science, University of Utah, Salt Lake City, UT, USA; Department of Physics and Astronomy, University of Utah, Salt Lake City, UT, USA.
Rodrigo GallegosCenter for Cell and Genome Science, University of Utah, Salt Lake City, UT, USA; Department of Physics and Astronomy, University of Utah, Salt Lake City, UT, USA.
Gabriel ClingerCenter for Cell and Genome Science, University of Utah, Salt Lake City, UT, USA; Department of Physics and Astronomy, University of Utah, Salt Lake City, UT, USA.
Nicole BohnCenter for Cell and Genome Science, University of Utah, Salt Lake City, UT, USA; Department of Physics and Astronomy, University of Utah, Salt Lake City, UT, USA.
Antje Huwendiek-PoserCenter for Cell and Genome Science, University of Utah, Salt Lake City, UT, USA; Department of Physics and Astronomy, University of Utah, Salt Lake City, UT, USA.
Gillian YsassiCenter for Cell and Genome Science, University of Utah, Salt Lake City, UT, USA; Department of Physics and Astronomy, University of Utah, Salt Lake City, UT, USA.
Allyson RomanCenter for Cell and Genome Science, University of Utah, Salt Lake City, UT, USA; Department of Physics and Astronomy, University of Utah, Salt Lake City, UT, USA; School of Biological Sciences, University of Utah, Salt Lake City, UT, USA.
Giovanna GarciaCenter for Cell and Genome Science, University of Utah, Salt Lake City, UT, USA; Department of Physics and Astronomy, University of Utah, Salt Lake City, UT, USA.
Tasheena ClyCenter for Cell and Genome Science, University of Utah, Salt Lake City, UT, USA; Department of Physics and Astronomy, University of Utah, Salt Lake City, UT, USA.
David BelnapSchool of Biological Sciences, University of Utah, Salt Lake City, UT, USA; Department of Biochemistry, University of Utah, Salt Lake City, UT, USA.
Saveez SaffarianCenter for Cell and Genome Science, University of Utah, Salt Lake City, UT, USA; Department of Physics and Astronomy, University of Utah, Salt Lake City, UT, USA; School of Biological Sciences, University of Utah, Salt Lake City, UT, USA; Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA, USA. Electronic address: saveez.saffarian@berkeley.edu.

Funding

Architecture and dynamics of immature HIV latticeR01AI186663 · NIAID · UTAH STATE HIGHER EDUCATION SYSTEM--UNIVERSITY OF UTAH · PI Saveez Saffarian, Michael D Vershinin · 2024 to 2026
$1.7M
NIAID NIH HHS R01 AI186663
6 · The paper itself

Abstract

HIV-1 is released from infected cells as immature virions whose membranes are supported by a Gag lattice. During maturation, this lattice is cleaved by the viral protease, releasing capsid proteins that assemble into the mature core. The architecture of the Gag lattice is central to this process, and the Gag lattice is targeted by maturation inhibitors that block cleavage. Using cryo-electron tomography, we compared Gag-only virus-like particles (VLPs) with immature HIV-1 virions and found that VLPs assemble denser, more complete lattices, with a strong correlation between lattice curvature and Gag copy number. In contrast, immature virions incorporate fewer Gag molecules and display weaker coupling between curvature and Gag stoichiometry. These findings show that while Gag alone can form the canonical immature lattice, additional viral components fine-tune lattice organization and curvature, potentially regulating protease accessibility, virion release, and the onset of HIV-1 maturation.

Indexed as

gag Gene Products, Human Immunodeficiency VirusHIV-1VirionCryoelectron MicroscopyElectron Microscope TomographyHumansModels, MolecularVirus Assemblygag Gene Products, Human Immunodeficiency Viruscryo-ETGag latticeGag VLPHIVimmature HIVlattice coverage

Identifiers

PMID41928506
PMCPMC13336345

What OpenQuestion holds

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Read underepoch 390

Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.