ArticleBMC genomics2026
Single-cell RNA and DNA methylome profiling reveal liquid helium vitrification enhances porcine parthenogenetically activated blastocyst viability.
Article in BMC genomics, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
0 citing papers in PubMed.
No citing paper in PubMed yet.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
9 authors.
Funding
Abstract
backgroundCryopreservation of porcine blastocysts remains a significant challenge due to their high lipid content, which can lead to cryoinjury and reduced developmental competence. While liquid nitrogen (LN) vitrification causes significant molecular damage, LHe vitrification enhances oocyte viability, reduces cryoinjury, and better preserves ultrastructure and gene expression. Despite these advantages, the specific effects of LHe vitrification on porcine blastocysts are still underexplored.
resultsLHe vitrification significantly improved post-thaw survival rates (96.53 ± 4.27%) compared to conventional LN (71.20 ± 6.82%) and modified LN protocols (90.77 ± 7.88%). scRNA-seq and scWGBS achieved mapping rates of 83.70% and 33.69%, respectively, and a bisulfite conversion efficiency of 99.27%, ensuring high-quality data for gene expression and methylation analysis. Vitrification significantly altered gene expression and epigenetic regulation, with hypermethylation and downregulation of genes associated with energy metabolism (ARG2, AUH, IDH3A, NDUFV1), immune function (CD53), and protein processing (B4GALT5, OSTC, DPM3, EXT1), resulting in mitochondrial and immune dysfunction, as well as protein instability. KEGG pathway analysis revealed disruptions in the TCA cycle, pentose phosphate pathway, homologous recombination, and fatty acid biosynthesis, which impaired blastocyst viability, ATP availability, immune responses, and cellular communication. Conversely, hypomethylation and upregulation of genes regulating energy metabolism (GPAM, AICDA), oxidative stress response (ITGAV), and cell adhesion (PLAU, MRAP2) suggest an adaptive response to mitigate cellular stress and damage. However, excessive upregulation following vitrification may lead to metabolic strain, oxidative stress accumulation, lipotoxicity, and an increased risk of apoptosis.
conclusionsOur findings demonstrate that LHe offers superior metabolic and epigenetic preservation compared to LN, thereby reducing molecular damage and enhancing post-thaw development. These results establish LHe vitrification as an effective method that increases blastocyst viability and reproductive biotechnology applications.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.