ArticleBMC molecular and cell biology2026
Adult canine pancreatic organoids enable functional analysis of pancreatic epithelial barrier and inflammatory responses.
Article in BMC molecular and cell biology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
0 citing papers in PubMed.
No citing paper in PubMed yet.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors.
Funding
Abstract
backgroundThree-dimensional (3D) organoid cultures from pancreatic tissue are emerging tools for studying pancreatic epithelial development, differentiation, and disease. While models derived from human fetal tissue or adult murine pancreas have provided key insights, their clinical translatability is limited by ethical and species-related differences. The domestic dog offers a valuable intermediate model for translational research, yet pancreatic organoid systems in this species remain underdeveloped.
resultsWe successfully established long-term organoid cultures from adult canine pancreas and demonstrated their stable ductal phenotype. These organoids exhibited limited but detectable expression of acinar and endocrine markers, indicating partial trilineage potential. We further developed two-dimensional (2D) monolayers from organoids and assessed epithelial barrier integrity using transepithelial electrical resistance (TEER). Monolayers maintained tight junctions and showed media-dependent changes in morphology and KRT19 expression. Peak TEER values exceeded 4000 Ω·cm², confirming robust epithelial barrier function.
conclusionsThis study presents a reproducible protocol for deriving organoids and monolayers from adult canine pancreas, offering a physiologically relevant and ethically accessible model for investigating pancreatic epithelial biology. The combination of 3D and 2D systems enables both lineage characterization and functional assessment, providing a foundation for future applications in regenerative medicine, disease modeling, and comparative translational research.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.