Evidence map›Paper›PMID 41927841›Full record

ArticleNature plants2026

Occupancy-based mechanism is the chief mode of ROS1 function in preventing DNA hypermethylation.

Li Deng, Guangfeng Zhu, Wenying Zhong, Zhibo Jia, Qiangwei Zhou, Minyan Zhang, Zhixin Si, Qing Zhang, Yafeng Liang, Xuan Du and 6 more

Abstract read
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In one paragraph

Article in Nature plants, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

16 authors.

Li Deng *National Key Laboratory of Crop Genetic Improvement, National Engineering Research Center of Rapeseed, Hubei Hongshan Laboratory, Huazhong Agricultural University, Wuhan, China.
Guangfeng Zhu *National Key Laboratory of Crop Genetic Improvement, National Engineering Research Center of Rapeseed, Hubei Hongshan Laboratory, Huazhong Agricultural University, Wuhan, China.
Wenying Zhong *National Key Laboratory of Crop Genetic Improvement, National Engineering Research Center of Rapeseed, Hubei Hongshan Laboratory, Huazhong Agricultural University, Wuhan, China.
Zhibo Jia *National Key Laboratory of Crop Genetic Improvement, National Engineering Research Center of Rapeseed, Hubei Hongshan Laboratory, Huazhong Agricultural University, Wuhan, China.
Qiangwei ZhouNational Key Laboratory of Crop Genetic Improvement, National Engineering Research Center of Rapeseed, Hubei Hongshan Laboratory, Huazhong Agricultural University, Wuhan, China.
Minyan ZhangNational Key Laboratory of Crop Genetic Improvement, National Engineering Research Center of Rapeseed, Hubei Hongshan Laboratory, Huazhong Agricultural University, Wuhan, China.
Zhixin SiNational Key Laboratory of Crop Genetic Improvement, National Engineering Research Center of Rapeseed, Hubei Hongshan Laboratory, Huazhong Agricultural University, Wuhan, China.
Qing ZhangNational Key Laboratory of Crop Genetic Improvement, National Engineering Research Center of Rapeseed, Hubei Hongshan Laboratory, Huazhong Agricultural University, Wuhan, China.
Yafeng LiangInstitute of Advanced Biotechnology and School of Medicine, Southern University of Science and Technology, Shenzhen, China.
Xuan DuGuangdong Provincial Key Laboratory for Plant Epigenetics, College of Life Sciences and Oceanography, Shenzhen University, Shenzhen, China.ORCID http://orcid.org/0000-0001-9589-3566
Yanfei MaoCenter for Excellence in Molecular Plant Sciences, Chinese Academy of Sciences, Shanghai, China.
Jian WuKey Laboratory of Plant Functional Genomics of the Ministry of Education, Yangzhou University, Yangzhou, China.
Guoliang LiNational Key Laboratory of Crop Genetic Improvement, National Engineering Research Center of Rapeseed, Hubei Hongshan Laboratory, Huazhong Agricultural University, Wuhan, China.ORCID http://orcid.org/0000-0003-1601-6640
Jinxiong ShenNational Key Laboratory of Crop Genetic Improvement, National Engineering Research Center of Rapeseed, Hubei Hongshan Laboratory, Huazhong Agricultural University, Wuhan, China.
Jian-Kang ZhuInstitute of Advanced Biotechnology and School of Medicine, Southern University of Science and Technology, Shenzhen, China. zhujk@sustech.edu.cn.ORCID http://orcid.org/0000-0001-5134-731X
Lun ZhaoNational Key Laboratory of Crop Genetic Improvement, National Engineering Research Center of Rapeseed, Hubei Hongshan Laboratory, Huazhong Agricultural University, Wuhan, China. zhaolun@mail.hzau.edu.cn.ORCID http://orcid.org/0000-0002-8065-9707

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

DNA demethylation is essential for maintaining genome-wide DNA methylation balance. Despite the substantial risk to genome stability, the prevailing paradigm posits that the Arabidopsis demethylase ROS1 prevents genome-wide DNA hypermethylation in vivo mainly through its 5-methylcytosine DNA glycosylase/lyase activity. Here we challenge this paradigm by demonstrating that ROS1, through its occupancy, drives extensive passive demethylation independent of its glycosylase/lyase activity and maintains hypomethylation primarily by preventing de novo DNA methylation. This occupancy-based mechanism eliminates the need for genome-wide base excision for active demethylation, thereby minimizing threats to genomic fidelity and stability. Beyond its role in demethylation, ROS1 also functions as a key marker and regulator of chromatin accessibility. This regulation operates in both DNA methylation-dependent and -independent contexts, with ROS1 acting as either a reserve or active protector of accessible chromatin, depending on the functional state of DNA methylation systems. Our findings redefine the diverse roles of ROS1 in DNA methylation regulation and chromatin accessibility, highlighting their intricate interplay.

Indexed as

ArabidopsisArabidopsis ProteinsDNA MethylationNuclear ProteinsChromatinArabidopsis ProteinsChromatinNuclear ProteinsROS1 protein, Arabidopsis

Identifiers

PMID41927841

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.