Protocol for automated quantification of neuronal cells using the Agilent BioTek Cytation system and Gen5 neurite outgrowth module.

Aline Broeglin, Olivier Buetzberger, Rebecca Mongeon, Aurélien Riou, Anne Eckert, Amandine Grimm

Abstract read

In one paragraph

Article in STAR protocols, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it

0cells of the map it votes in

0citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Aline BroeglinResearch Cluster, Molecular & Cognitive Neuroscience, Department of Biomedicine, University of Basel, 4001 Basel, Switzerland. Electronic address: aline.broeglin@unibas.ch.

Olivier BuetzbergerAgilent Technologies, EPFL Innovation Park, 1015 Lausanne, Switzerland.

Rebecca MongeonAgilent Technologies, Winooski, VT 05404, USA.

Aurélien RiouResearch Cluster, Molecular & Cognitive Neuroscience, Department of Biomedicine, University of Basel, 4001 Basel, Switzerland.

Anne EckertNeurobiology Laboratory for Brain Aging and Mental Health, University Psychiatric Clinics (UPK), 4002 Basel, Switzerland.

Amandine GrimmResearch Cluster, Molecular & Cognitive Neuroscience, Department of Biomedicine, University of Basel, 4001 Basel, Switzerland; Neurobiology Laboratory for Brain Aging and Mental Health, University Psychiatric Clinics (UPK), 4002 Basel, Switzerland. Electronic address: amandine.grimm@unibas.ch.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Drugs or pathological proteins can influence neuronal plasticity. Here, we provide a protocol to quantify neurite outgrowth parameters in differentiated SH-SY5Y neuroblastoma cells using the Agilent BioTek Gen5 neurite outgrowth module. The protocol includes steps for plating cells, inducing differentiation, performing immunostaining, collecting images automatically, and analyzing neurite morphology. This assay enables the evaluation of neuronal plasticity and assesses the effects of drugs or pathological proteins. It can be adapted to other neuronal models and scaled for high-throughput screening.

Indexed as

NeuritesNeuronal OutgrowthNeuronsCell DifferentiationCell Line, TumorHigh-Throughput Screening AssaysHumansCell BiologyHigh Throughput ScreeningMicroscopyNeuroscience

Identifiers

PMID41926289

DOI10.1016/j.xpro.2026.104485

PMCPMC13068831

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.