Protocol for isolating and culturing microglia from the adult mouse brain using a magnetic-activated cell sorting system.

Joohyun Park, Bhabotosh Barman, Hyun Kyu Kim, Fuzheng Guo

Abstract read

In one paragraph

Article in STAR protocols, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it

0cells of the map it votes in

0citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Joohyun ParkDepartment of Neurology, School of Medicine, University of California, Davis, Stockton Blvd, Sacramento, CA 95817, USA; Institute of Pediatric Reparative Research (IPRM), Shriners Hospital for Children, Sacramento, CA 95817, USA. Electronic address: pjhpark@health.ucdavis.edu.

Bhabotosh BarmanDepartment of Neurology, School of Medicine, University of California, Davis, Stockton Blvd, Sacramento, CA 95817, USA; Institute of Pediatric Reparative Research (IPRM), Shriners Hospital for Children, Sacramento, CA 95817, USA.

Hyun Kyu KimDepartment of Neurology, School of Medicine, University of California, Davis, Stockton Blvd, Sacramento, CA 95817, USA; Institute of Pediatric Reparative Research (IPRM), Shriners Hospital for Children, Sacramento, CA 95817, USA.

Fuzheng GuoDepartment of Neurology, School of Medicine, University of California, Davis, Stockton Blvd, Sacramento, CA 95817, USA; Institute of Pediatric Reparative Research (IPRM), Shriners Hospital for Children, Sacramento, CA 95817, USA. Electronic address: fzguo@health.ucdavis.edu.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Microglia are the resident macrophages of the central nervous system and are highly sensitive to environmental cues, making primary culture challenging. Here, we present a protocol for isolating microglia from adult mouse brains using magnetic-activated cell sorting (MACS). The protocol includes brain sample collection, enzymatic tissue dissociation, step-by-step MACS-based microglia isolation, and the establishment of primary microglial cultures. We validate isolated cells using flow cytometry and quantitative real-time PCR (real-time qPCR) to assess microglial purity.

Indexed as

BrainCell Culture TechniquesCell SeparationFlow CytometryImmunomagnetic SeparationMicrogliaAnimalsCells, CulturedMiceCell-based AssaysCell cultureCell isolationCell separation/fractionationNeuroscience

Identifiers

PMID41926288

DOI10.1016/j.xpro.2026.104471

PMCPMC13068824

What OpenQuestion holds

Textmetadata

LicenceCC BY-NC-ND

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.