Evidence map›Paper›PMID 41925969›Full record

ArticleBrazilian journal of microbiology : [publication of the Brazilian Society for Microbiology]2026

Decoding the genome of Bacillus thuringiensis subsp. israelensis VCRC B668 to understand its mosquitocidal potential for biopesticide development.

Jibi Lukose, A Mathivanan, Bhavna Gupta, Sahadiya Mandodan, V Abhisubesh, Kakhuangailiu Gangmei, Bhagyashree Bora, Aneha Kunnikuruvan, P Hemaladkshmi, I Geetha and 2 more

Abstract read
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In one paragraph

Article in Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology], 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors.

Jibi LukoseDivision of Microbiology, ICMR- Vector Control Research Centre, Affiliated to Pondicherry University, Puducherry, 605006, India.
A MathivananDivision of Microbiology, ICMR- Vector Control Research Centre, Affiliated to Pondicherry University, Puducherry, 605006, India.
Bhavna GuptaICMR- Vector Control Research Centre, Field station, Madurai, Tamil Nadu, 625002, India.
Sahadiya MandodanDivision of Microbiology, ICMR- Vector Control Research Centre, Affiliated to Pondicherry University, Puducherry, 605006, India.
V AbhisubeshDivision of Microbiology, ICMR- Vector Control Research Centre, Affiliated to Pondicherry University, Puducherry, 605006, India.
Kakhuangailiu GangmeiDivision of Microbiology, ICMR- Vector Control Research Centre, Affiliated to Pondicherry University, Puducherry, 605006, India.
Bhagyashree BoraDivision of Microbiology, ICMR- Vector Control Research Centre, Affiliated to Pondicherry University, Puducherry, 605006, India.
Aneha KunnikuruvanDivision of Microbiology, ICMR- Vector Control Research Centre, Affiliated to Pondicherry University, Puducherry, 605006, India.
P HemaladkshmiDivision of Microbiology, ICMR- Vector Control Research Centre, Affiliated to Pondicherry University, Puducherry, 605006, India.
I GeethaDivision of Microbiology, ICMR- Vector Control Research Centre, Affiliated to Pondicherry University, Puducherry, 605006, India.
S PoopathiDivision of Microbiology, ICMR- Vector Control Research Centre, Affiliated to Pondicherry University, Puducherry, 605006, India.
R ParamasivanICMR- Vector Control Research Centre, Field station, Madurai, Tamil Nadu, 625002, India. rpsivan2000@gmail.com.ORCID http://orcid.org/0000-0001-5262-2301

Funding

DST-INSPIRE Fellowship (IF210310)
6 · The paper itself

Abstract

Whole-genome sequencing (WGS) was conducted to characterize the potent mosquitocidal bacterium Bacillus thuringiensis subsp. israelensis VCRC B668, isolated from soil in Tamil Nadu, India. Sequencing using the Illumina MiSeq platform produced a 6.25 Mb genome with 34.97% GC content, 6,441 coding sequences, 115 tRNAs, and 16 rRNAs. Annotation and analysis through RAST, Prokka, and BV-BRC confirmed its close relation to Bti strains AM65-52 and BGSC 4Q1 based on ANI and dDDH values. Seven plasmids were detected, including one resembling pBtoxis carrying major cry and cyt toxin genes. Sixteen insecticidal genes were identified, including cry4Aa, cry4Ba, cry11Aa, and cyt1Aa. SDS–PAGE analysis revealed expression of protein bands at approximately 130 kDa, 70 kDa, and 25 kDa, which were subsequently identified by LC–MS/MS as Cry4Ba, Cry11Aa, and Cyt1Aa, respectively. Bioassays confirmed high larvicidal activity against Aedes aegypti, Culex quinquefasciatus, and Anopheles stephensi, with LC₅₀ values ranging from 0.0076 to 0.0098 mg/L, highlighting its strong biocontrol potential.

Indexed as

Bacillus thuringiensisBiological Control AgentsGenome, BacterialInsecticidesAedesAnimalsAnophelesBacillus thuringiensis ToxinsBacterial ProteinsCulexEndotoxinsHemolysin ProteinsIndiaLarvaPest Control, BiologicalSoil MicrobiologyBacillus thuringiensis ToxinsBacterial ProteinsBiological Control AgentsEndotoxinsHemolysin Proteinsinsecticidal crystal protein, Bacillus ThuringiensisInsecticidesBacillus thuringiensis subsp. israelensisLC-MS/MSProtein analysisSDS-PAGEWhole genome sequence

Identifiers

PMID41925969
PMCPMC13047025

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.