Evidence map›Paper›PMID 41925918›Full record

ArticlePlant cell reports2026

A nonspecific lipid transfer protein, CtnsLTP18, mediates drought stress resistance in Cynanchum thesioides.

Xiaoyao Chang, Xiaoyan Zhang, Xiumei Huang, Zhongren Yang, Guihua Chen, Fenglan Zhang

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Article in Plant cell reports, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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4 · The record

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5 · Who and what money

Authors and funding

6 authors.

Xiaoyao ChangInner Mongolia Key Laboratory of Wild Peculiar Vegetable Germplasm Resource and Germplasm Enhancement, College of Horticultural and Plant Protection, Inner Mongolia Agricultural University, Inner Mongolia, Huhhot, China.
Xiaoyan ZhangInner Mongolia Key Laboratory of Wild Peculiar Vegetable Germplasm Resource and Germplasm Enhancement, College of Horticultural and Plant Protection, Inner Mongolia Agricultural University, Inner Mongolia, Huhhot, China.
Xiumei HuangDepartment of Horticulture and Landscape Technology, Inner Mongolia Agricultural University Vocational and Technical College, Inner Mongolia, Baotou City, China.
Zhongren YangInner Mongolia Key Laboratory of Wild Peculiar Vegetable Germplasm Resource and Germplasm Enhancement, College of Horticultural and Plant Protection, Inner Mongolia Agricultural University, Inner Mongolia, Huhhot, China.
Guihua ChenInner Mongolia Key Laboratory of Wild Peculiar Vegetable Germplasm Resource and Germplasm Enhancement, College of Horticultural and Plant Protection, Inner Mongolia Agricultural University, Inner Mongolia, Huhhot, China.
Fenglan ZhangInner Mongolia Key Laboratory of Wild Peculiar Vegetable Germplasm Resource and Germplasm Enhancement, College of Horticultural and Plant Protection, Inner Mongolia Agricultural University, Inner Mongolia, Huhhot, China. zhangfenglan041105@163.com.

Funding

Major Science and Technology Special Program of Inner Mongolia Autonomous Region No. 2021ZD0001Natural Science Foundation of Inner Mongolia Autonomous Region No. 2025QN03142Science and Technology Program of Inner Mongolia Autonomous Region No. 2021GG0084the Basic research funds for universities directly under the Inner Mongolia Autonomous Region BR22-11-06the Inner Mongolia Autonomous Region Scientific and Technological Achievements Transformation Special Fund Project No. 2021CG0023
6 · The paper itself

Abstract

key messageThis study first reveals the CtBBX4-CtnsLTP18-CtIPCS1 module mediates drought tolerance via the antioxidant system in Cynanchum thesioides, off ering genetic resources for plant abiotic stress improvement. Cynanchum thesioides, a wild plant with both medicinal and edible values widely distributed in Inner Mongolia of China, exhibits excellent adaptability to arid environments. However, the regulatory network of its nonspecific lipid transfer protein (nsLTP) gene family in drought response remains elusive. In this study, we preliminarily identified the CtnsLTP gene family in C. thesioides and conducted an in-depth analysis focusing on CtnsLTP18. Phylogenetic analysis revealed that the CtnsLTP gene family comprises 18 structurally divergent members, which are clustered into 9 subfamilies. CtnsLTP18 was significantly upregulated at the early stage of drought stress, and its encoded protein was localized to the plasma membrane. Functional validation assays showed that overexpression of CtnsLTP18 in Saccharomyces cerevisiae enhanced its osmotic stress tolerance, while heterologous overexpression of this gene in Arabidopsis thaliana improved the drought tolerance of transgenic plants. In contrast, suppression of CtnsLTP18 expression in C. thesioides via virus-induced gene silencing (VIGS) resulted in a significant reduction in plant drought resistance. At the physiological level, CtnsLTP18 enhanced plant drought resistance by elevating the activity of antioxidant enzymes, reducing reactive oxygen species (ROS) accumulation and alleviating membrane lipid peroxidation damage. At the molecular level, yeast two-hybrid (Y2H), bimolecular fluorescence complementation (BiFC), and luciferase complementation assay (LCA) experiments confirmed a physical interaction between CtnsLTP18 and CtIPCS1. Furthermore, yeast one-hybrid (Y1H), electrophoretic mobility shift assay (EMSA), and dual-luciferase reporter assays demonstrated that the transcription factor CtBBX4 could directly bind to the promoter of CtnsLTP18 and positively regulate its expression. Taken together, this study is the first to elucidate the molecular mechanism by which the CtBBX4-CtnsLTP18-CtIPCS1 regulatory module mediates drought tolerance in C. thesioides through the modulation of the antioxidant system. This finding not only enriches our understanding of the drought adaptation strategies of C. thesioides but also provides valuable genetic resources for the abiotic stress-resistant genetic improvement of plants.

Indexed as

Carrier ProteinsCynanchumPlant ProteinsArabidopsisDrought ResistanceDroughtsGene Expression Regulation, PlantPhylogenyPlants, Genetically ModifiedSaccharomyces cerevisiaeStress, PhysiologicalCarrier Proteinslipid transfer proteinPlant ProteinsAntioxidant systemCtnsLTP18Cynanchum thesioidesDrought toleranceNonspecific lipid transfer protein

Identifiers

PMID41925918

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.