Evidence map›Paper›PMID 41925227›Full record

ArticleThe ISME journal2026

Microoxic conditions promote Escherichia-associated cellulase expression in the giant panda gut.

Feilong Deng, Yanhua Han, Yunjuan Peng, Zhijian Xu, Jianbo Yang, Jinling He, Desheng Li, Guixin Dong, Peng Zhang, Hui Jiang and 4 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in The ISME journal, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Strain-resolved biology ofFrontiers in veterinary science · 2026
    Review
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

14 authors.

Feilong DengGuangdong Provincial Key Laboratory of Animal Molecular Design and Precise Breeding, School of Animal Science and Technology, Foshan University, Foshan 528225, China.
Yanhua HanGuangdong Provincial Key Laboratory of Animal Molecular Design and Precise Breeding, School of Animal Science and Technology, Foshan University, Foshan 528225, China.
Yunjuan PengCollege of Animal Science, South China Agricultural University, Guangzhou 510642, China.
Zhijian XuGuangdong Provincial Key Laboratory of Animal Molecular Design and Precise Breeding, School of Animal Science and Technology, Foshan University, Foshan 528225, China.
Jianbo YangGuangdong Provincial Key Laboratory of Animal Molecular Design and Precise Breeding, School of Animal Science and Technology, Foshan University, Foshan 528225, China.
Jinling HeGuangdong Provincial Key Laboratory of Animal Molecular Design and Precise Breeding, School of Animal Science and Technology, Foshan University, Foshan 528225, China.
Desheng LiChina Conservation and Research Center for the Giant Panda, Key Laboratory of SFGA on the Giant Panda, Chengdu 611830, China.
Guixin DongSouthern China Wildlife Species Conservation Center, Zhuhai 519031, China.
Peng ZhangSouthern China Wildlife Species Conservation Center, Zhuhai 519031, China.
Hui JiangGuangdong Provincial Key Laboratory of Animal Molecular Design and Precise Breeding, School of Animal Science and Technology, Foshan University, Foshan 528225, China.
Jianmin ChaiGuangdong Provincial Key Laboratory of Animal Molecular Design and Precise Breeding, School of Animal Science and Technology, Foshan University, Foshan 528225, China.
Chengdong WangChina Conservation and Research Center for the Giant Panda, Key Laboratory of SFGA on the Giant Panda, Chengdu 611830, China.
Jiangchao ZhaoCollege of Animal Science, South China Agricultural University, Guangzhou 510642, China.
Ying LiGuangdong Provincial Key Laboratory of Animal Molecular Design and Precise Breeding, School of Animal Science and Technology, Foshan University, Foshan 528225, China.

Funding

National Natural Science Foundation of China 32170430National Natural Science Foundation of China 32400412Specific University Discipline Construction Project 2023B10564001
6 · The paper itself

Abstract

Giant pandas possess a carnivore-like gastrointestinal tract yet subsist on bamboo, and their gut communities contain few canonical cellulolytic taxa. We investigated how fiber processing proceeds in this setting by building a species-resolved reference and linking community features to cellular transcriptional profiles and isolate phenotypes. Using culturomics and PacBio HiFi metagenomics, we assembled a species-resolved reference catalog for the panda gut microbiome (Pbac v2; 466 species-level genomes). Community profiling across 142 samples resolved three enterotypes dominated by Escherichia coli (ET-Ecoli), Clostridium SGBP116 (ET-Clos), and Streptococcus alactolyticus (ET-StreA), with ET-Ecoli enriched for tricarboxylic-acid and respiratory-chain modules and showing higher abundance of an endo-β-1,4-glucanase marker. Droplet-based microbial single-cell RNA-seq from four samples (16 659 cells) assigned a substantial share of cellulase-associated transcripts (GH1/GH3/GH5/GH9) in situ to Escherichia and revealed within-species heterogeneity: E. coli subpopulations segregated into respiration-enriched versus three-carbon/anaerobic-like programs, with cellulase/lytic polysaccharide monooxygenase-linked transcripts concentrated in the former. Guided by these associations, panda-derived E. coli isolates assayed under defined atmospheres showed oxygen-dependent cellulolytic readouts in vitro. Although in vivo oxygen levels were not measured, the convergence of species-resolved community signatures, single-cell attribution, and isolate phenotypes indicates that E. coli can contribute to cellulose processing under microoxic conditions in this cohort. The Pbac v2 resource and the integrated workflow (culturomics + HiFi metagenomes, multi-omics, microbial scRNA-seq) provide a template for species-level assignment of microbiome functions in hosts with unconventional diet-physiology combinations.

Indexed as

CellulaseEscherichia coliGastrointestinal MicrobiomeUrsidaeAnimalsMetagenomicsCellulaseEscherichia coligiant panda gut microbiotahost-microbe coadaptationmicrobial scRNA-seq

Identifiers

PMID41925227
PMCPMC13099265

What OpenQuestion holds

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LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.