ArticleJournal of experimental pharmacology2026
Development and Validation of Bioanalytical Method for Efavirenz in Dried Blood Spot Using High Performance Liquid Chromatography-Photodiode Array.
Article in Journal of experimental pharmacology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
0 citing papers in PubMed.
No citing paper in PubMed yet.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Efavirenz requires adequate serum concentrations (1-4 µg/mL) to ensure therapeutic efficacy and avoid central nervous system toxicity. Due to significant interindividual pharmacokinetic variability, therapeutic drug monitoring (TDM) is often necessary. Reliable bioanalytical methods for quantifying efavirenz in biological matrices are therefore essential. Previous dried blood spot (DBS) methods using High-Performance Liquid Chromatography-Photodiode Array (HPLC-PDA) showed suboptimal performance and lacked compliance with current bioanalytical guidelines. This study aimed to develop and validate an improved HPLC-PDA method for efavirenz quantification in DBS using an internal standard. The optimized sample preparation employed protein precipitation and methanol extraction, followed by chromatographic separation under isocratic conditions with UV detection at 245 nm. The method demonstrated excellent linearity
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.