Evidence map›Paper›PMID 41923624›Full record

ArticleCell reports. Medicine2026

Human promoter-driven AAV tools enable precision gene therapy targeting cochlear hair cells.

Liyan Zhang, Yicheng Lu, Xianmin Wu, Jinyi Fan, Xinru Zhang, Yang Xu, Hao Wei, Qiuhan Sun, Lulu Jiang, Huawei Tuo and 11 more

Abstract read
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Article in Cell reports. Medicine, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

What it found

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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3 · Its place in the literature

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No citing paper in PubMed yet.

4 · The record

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PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

21 authors.

Liyan ZhangState Key Laboratory of Digital Medical Engineering, Department of Otolaryngology Head and Neck Surgery, Zhongda Hospital, School of Life Sciences and Technology, School of Medicine, Advanced Institute for Life and Health, Jiangsu Province High-Tech Key Laboratory for Bio-Medical Research, Southeast University, Nanjing, China; Department of Anatomy the Basic Medicine College, Inner Mongolia Medical University, Hohhot, China.
Yicheng LuState Key Laboratory of Digital Medical Engineering, Department of Otolaryngology Head and Neck Surgery, Zhongda Hospital, School of Life Sciences and Technology, School of Medicine, Advanced Institute for Life and Health, Jiangsu Province High-Tech Key Laboratory for Bio-Medical Research, Southeast University, Nanjing, China.
Xianmin WuState Key Laboratory of Digital Medical Engineering, Department of Otolaryngology Head and Neck Surgery, Zhongda Hospital, School of Life Sciences and Technology, School of Medicine, Advanced Institute for Life and Health, Jiangsu Province High-Tech Key Laboratory for Bio-Medical Research, Southeast University, Nanjing, China.
Jinyi FanState Key Laboratory of Digital Medical Engineering, Department of Otolaryngology Head and Neck Surgery, Zhongda Hospital, School of Life Sciences and Technology, School of Medicine, Advanced Institute for Life and Health, Jiangsu Province High-Tech Key Laboratory for Bio-Medical Research, Southeast University, Nanjing, China.
Xinru ZhangState Key Laboratory of Digital Medical Engineering, Department of Otolaryngology Head and Neck Surgery, Zhongda Hospital, School of Life Sciences and Technology, School of Medicine, Advanced Institute for Life and Health, Jiangsu Province High-Tech Key Laboratory for Bio-Medical Research, Southeast University, Nanjing, China.
Yang XuDepartment of Biomedical Engineering, Southern University of Science and Technology, Shenzhen, China.
Hao WeiDepartment of Otolaryngology Head and Neck Surgery, Nanjing Drum Tower Hospital, Affiliated Hospital of Medical School, Nanjing University, Jiangsu Provincial Key Medical Discipline (Laboratory), Nanjing, China; Research Institute of Otolaryngology, Nanjing, China.
Qiuhan SunState Key Laboratory of Digital Medical Engineering, Department of Otolaryngology Head and Neck Surgery, Zhongda Hospital, School of Life Sciences and Technology, School of Medicine, Advanced Institute for Life and Health, Jiangsu Province High-Tech Key Laboratory for Bio-Medical Research, Southeast University, Nanjing, China.
Lulu JiangOtovia Therapeutics Inc., Suzhou, China.
Huawei TuoState Key Laboratory of Digital Medical Engineering, Department of Otolaryngology Head and Neck Surgery, Zhongda Hospital, School of Life Sciences and Technology, School of Medicine, Advanced Institute for Life and Health, Jiangsu Province High-Tech Key Laboratory for Bio-Medical Research, Southeast University, Nanjing, China.
Nianci LiState Key Laboratory of Digital Medical Engineering, Department of Otolaryngology Head and Neck Surgery, Zhongda Hospital, School of Life Sciences and Technology, School of Medicine, Advanced Institute for Life and Health, Jiangsu Province High-Tech Key Laboratory for Bio-Medical Research, Southeast University, Nanjing, China.
Hua JinState Key Laboratory of Digital Medical Engineering, Department of Otolaryngology Head and Neck Surgery, Zhongda Hospital, School of Life Sciences and Technology, School of Medicine, Advanced Institute for Life and Health, Jiangsu Province High-Tech Key Laboratory for Bio-Medical Research, Southeast University, Nanjing, China.
Wu CaoDepartment of Otorhinolaryngology-Head and Neck Surgery, Zhongda Hospital, Medicine School, Southeast University, Nanjing, China.
Beibei YeDepartment of Otorhinolaryngology-Head and Neck Surgery, Zhongda Hospital, Medicine School, Southeast University, Nanjing, China.
Ziyu ZhangState Key Laboratory of Digital Medical Engineering, Department of Otolaryngology Head and Neck Surgery, Zhongda Hospital, School of Life Sciences and Technology, School of Medicine, Advanced Institute for Life and Health, Jiangsu Province High-Tech Key Laboratory for Bio-Medical Research, Southeast University, Nanjing, China.
Yunkai ZhangState Key Laboratory of Digital Medical Engineering, Department of Otolaryngology Head and Neck Surgery, Zhongda Hospital, School of Life Sciences and Technology, School of Medicine, Advanced Institute for Life and Health, Jiangsu Province High-Tech Key Laboratory for Bio-Medical Research, Southeast University, Nanjing, China.
Fangyi ChenDepartment of Biomedical Engineering, Southern University of Science and Technology, Shenzhen, China.
Ling LuDepartment of Otorhinolaryngology-Head and Neck Surgery, Zhongda Hospital, Medicine School, Southeast University, Nanjing, China. Electronic address: entluling60@126.com.
Jieyu QiDepartment of Radiology, Zhuhai People's Hospital, The Affiliated Hospital of Beijing Institute of Technology, Advanced Technology Research Institute, School of Life Science, Beijing Institute of Technology, Beijing, China; State Key Laboratory of Hearing and Balance Science, Beijing Institute of Technology, Beijing, China; Advanced Technology Research Institute, Beijing Institute of Technology, Jinan, China. Electronic address: qijieyu@bit.edu.cn.
Renjie ChaiState Key Laboratory of Digital Medical Engineering, Department of Otolaryngology Head and Neck Surgery, Zhongda Hospital, School of Life Sciences and Technology, School of Medicine, Advanced Institute for Life and Health, Jiangsu Province High-Tech Key Laboratory for Bio-Medical Research, Southeast University, Nanjing, China; Department of Radiology, Zhuhai People's Hospital, The Affiliated Hospital of Beijing Institute of Technology, Advanced Technology Research Institute, School of Life Science, Beijing Institute of Technology, Beijing, China; Co-Innovation Center of Neuroregeneration, Nantong University, Nantong, China; Department of Otolaryngology Head and Neck Surgery, Sichuan Provincial People's Hospital, School of Medicine, University of Electronic Science and Technology of China, Chengdu, China; Southeast University Shenzhen Research Institute, Shenzhen, China. Electronic address: renjiec@seu.edu.cn.
Fangzhi TanState Key Laboratory of Digital Medical Engineering, Department of Otolaryngology Head and Neck Surgery, Zhongda Hospital, School of Life Sciences and Technology, School of Medicine, Advanced Institute for Life and Health, Jiangsu Province High-Tech Key Laboratory for Bio-Medical Research, Southeast University, Nanjing, China. Electronic address: 101013491@seu.edu.cn.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Approximately 50% of hereditary deafness cases and most age-related hearing loss result from cochlear hair cell dysfunction. Adeno-associated virus (AAV)-mediated gene therapy offers a promising strategy for hearing restoration. However, the cochlea's complex cellular composition poses a significant challenge for targeted gene delivery into hair cells. Here, we establish a cross-species screening platform for cochlear AAV tools, applicable from rodents to large animals. Using this platform, we identify human promoters that specifically target inner hair cells, outer hair cells, or all hair cells in mice. The hair cell-specific coProB2 promoter is further validated in Bama miniature pigs and cynomolgus monkeys, achieving specific transgene expression in hair cells without auditory toxicity. Importantly, coProB2-driven gene therapies in DFNB79 and DFNB9 mouse models restore auditory function to near wild-type levels. Thus, this study establishes a cross-species-compatible, hair cell-specific AAV system, providing a versatile toolbox for cochlear hair cell gene therapy.

Indexed as

DependovirusGenetic TherapyHair Cells, AuditoryPromoter Regions, GeneticAnimalsDisease Models, AnimalGene Therapy AgentsGenetic VectorsHumansMacaca fascicularisMiceSwineSwine, MiniatureTransgenesAAVcochlear hair cellsdeafnessDFNB79DFNB9gene therapyhearing losshuman-derived specific promoter

Identifiers

PMID41923624
PMCPMC13130653

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.