Evidence map›Paper›PMID 41923221›Full record

ArticleBMC plant biology2026

Integrated metabolome and transcriptome analyses reveal the role played by CaWRKY44 during anthocyanins accumulation in pepper fruits.

Qiaoling Deng, Zhengcong Luo, Guoping Zhang, Yuping Bai, Wei Huang, Guining Fang, Xuewen Chen, Mengjuan Li, Qiaoli Xie, Minghua Deng and 1 more

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Article in BMC plant biology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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5 · Who and what money

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11 authors.

Qiaoling Deng *Key Laboratory of Vegetable Biology of Yunnan Province, College of Landscape and Horticulture, Yunnan Agricultural University, Kunming, 650201, China.
Zhengcong Luo *Key Laboratory of Vegetable Biology of Yunnan Province, College of Landscape and Horticulture, Yunnan Agricultural University, Kunming, 650201, China.
Guoping Zhang *Key Laboratory of Vegetable Biology of Yunnan Province, College of Landscape and Horticulture, Yunnan Agricultural University, Kunming, 650201, China.
Yuping BaiKey Laboratory of Vegetable Biology of Yunnan Province, College of Landscape and Horticulture, Yunnan Agricultural University, Kunming, 650201, China.
Wei HuangKey Laboratory of Vegetable Biology of Yunnan Province, College of Landscape and Horticulture, Yunnan Agricultural University, Kunming, 650201, China.
Guining FangKey Laboratory of Vegetable Biology of Yunnan Province, College of Landscape and Horticulture, Yunnan Agricultural University, Kunming, 650201, China.
Xuewen ChenKey Laboratory of Vegetable Biology of Yunnan Province, College of Landscape and Horticulture, Yunnan Agricultural University, Kunming, 650201, China.
Mengjuan LiKey Laboratory of Vegetable Biology of Yunnan Province, College of Landscape and Horticulture, Yunnan Agricultural University, Kunming, 650201, China.
Qiaoli XieCollege of Bioengineering, Chongqing University, Chongqing, 400044, China.
Minghua DengKey Laboratory of Vegetable Biology of Yunnan Province, College of Landscape and Horticulture, Yunnan Agricultural University, Kunming, 650201, China. 2003023@ynau.edu.cn.
Junheng LvKey Laboratory of Vegetable Biology of Yunnan Province, College of Landscape and Horticulture, Yunnan Agricultural University, Kunming, 650201, China. junhenglv@ynau.edu.cn.

Funding

National Natural Science Foundation of China 32160708, 32360744Project of Yunnan Science and Technology Plan 202502AE090018，202202AE090031, 202205AR070001, 202301BD070001-016"Xingdian talent supportplan" of Yunnan Province yfgrc202424，XDYC-QNRC-2022-0713
6 · The paper itself

Abstract

backgroundPepper is an important vegetable and widely cultivated across the world. Anthocyanins play a crucial role in determining the color and nutritional value of pepper. In this study, light effects on anthocyanins accumulation in purple peppers were analyzed via bagging and non-bagging treatments.

resultsTranscription factor CaWRKY44 was identified as playing a key role in anthocyanins synthesis according to the metabolome and transcriptome analysis. CaWRKY44 expression increased during the different pepper pericarp developmental stages and a promoter cis-element analysis identified light and hormone-responsive elements and protein-binding motifs. VIGS-mediated silencing of CaWRKY44 reduced anthocyanins accumulation, while transient overexpression enhanced pigment deposition. Furthermore, the silencing or overexpression of CaWRKY44 modulated the expression level of anthocyanins biosynthetic structural genes.

conclusionsOur results indicated that CaWRKY44 could directly bind to the W-box cis-element in the CaDFR promoter and activates its transcription, which subsequently drives anthocyanins biosynthesis. The results could lay the foundation of the regulatory mechanism controlling anthocyanins biosynthesis in pepper.

Indexed as

AnthocyaninsCapsicumFruitMetabolomePlant ProteinsTranscription FactorsGene Expression ProfilingGene Expression Regulation, PlantPromoter Regions, GeneticTranscriptomeAnthocyaninsPlant ProteinsTranscription FactorsAnthocyaninsMetabolome and transcriptomePepperRegulatory mechanismWRKY transcription factor

Identifiers

PMID41923221
PMCPMC13169654

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