ArticleBMC microbiology2026
Mechanistic insights into pregnenolone-mediated conidiation inhibition in Fusarium oxysporum.
Article in BMC microbiology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
0 citing papers in PubMed.
No citing paper in PubMed yet.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundFusarium oxysporum (FO) is a plant pathogen that causes significant economic losses worldwide. Conidia are the primary source of FO infection and survival till the next generation. Hence, controlling conidiation is an important goal in plant protection research.
methodsThe Fusarium oxysporum LZ51 was isolated from infected tomato plants and identified through ITS sequencing. Shenzhen BGI Co., Ltd. performed RNA sequencing using the BGI Multiomics System. F. oxysporum genes were knocked out via homologous recombination. ERG2 and EBP GFP-tagged proteins were synthesized in E. coli BL21 (DE3). The expressed proteins were subjected to Microscale Thermophoresis (MST) analysis.
resultsThe present study investigated the efficacy of pregnenolone as a conidiation inhibitor against Fusarium oxysporum. It was determined that pregnenolone (Preg) effectively inhibited conidiation of Fusarium oxysporum, with an inhibition rate of ~ 42% at 10 mg/L. Furthermore, RNA-seq analysis identified (5) upregulated genes in the steroid biosynthesis pathway, suggesting that pregnenolone regulates conidia production through this pathway. Knocking out the steroid biosynthesis genes ERG4, ERG2, FDFT1, EBP, and WOR2 significantly decreased conidial production. HPLC analysis showed significantly lower ergosterol content in all the FO mutants. Molecular docking results showed that ERG2 and EBP have higher binding affinity to pregnenolone as compared to other genes. Further analysis predicted that pregnenolone binds to both ERG2 and EBP.
conclusionThese findings validate ERG2 and EBP as molecular targets of pregnenolone, suggesting their roles in inhibiting fungal conidiation.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.