Evidence map›Paper›PMID 41920742›Full record

ArticleSTAR protocols2026

Protocol for the isolation, culturing, and evaluation of primary Müller glial cells from pig retina as cellular models in health and disease.

Yesim Tütüncü, Jan Motlik, Nikolai Klymiuk, Uwe Wolfrum

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in STAR protocols, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Yesim TütüncüInstitute of Molecular Physiology, Molecular Cell Biology, Johannes Gutenberg University Mainz, 55128 Mainz, Germany.
Jan MotlikInstitute of Animal Physiology and Genetics, Czech Academy of Sciences, 27721 Libechov, Czech Republic.
Nikolai KlymiukLarge Animal Models in Cardiovascular Research, Internal Medical Department I, Technical University of Munich, 81675 Munich, Germany.
Uwe WolfrumInstitute of Molecular Physiology, Molecular Cell Biology, Johannes Gutenberg University Mainz, 55128 Mainz, Germany; Institute of Quantitative and Computational Biosciences (IQCB), Johannes GutenbergUniversity of Mainz, 55128 Mainz, Germany. Electronic address: wolfrum@uni-mainz.de.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Müller glial cells maintain retinal homeostasis and provide structural and metabolic support. The pig eye closely resembles the human eye in morphology and physiology, offering strong translational potential. Here, we present a protocol for isolating and culturing primary Müller glial cells from pig retina to study their function in health and disease. We describe steps for eyeball dissection, retinal isolation, photoreceptor cell depletion, and retinal digestion. We then detail procedures for the selection, culture, and characterization of Müller glial cells.

Indexed as

Cell Culture TechniquesCell SeparationEpendymoglial CellsRetinaAnimalsCells, CulturedSwineCell BiologyCell cultureCell isolationNeuroscience

Identifiers

PMID41920742
PMCPMC13068851

What OpenQuestion holds

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LicenceCC BY-NC-ND
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.