Simplified In Vitro Assay for Testing Chemicals with Auxin Activity Using Direct Protein-Protein Interaction.

Heshini N Weerakkody, Ikechukwu E Opara, Prianka Acharjya, Sunethra Dharmasiri, Nihal Dharmasiri

Abstract read

In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it

0cells of the map it votes in

0citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Heshini N WeerakkodyDepartment of Biology, Texas State University, San Marcos, TX, USA.

Ikechukwu E OparaDepartment of Biology, Texas State University, San Marcos, TX, USA.

Prianka AcharjyaDepartment of Biology, Texas State University, San Marcos, TX, USA.

Sunethra DharmasiriDepartment of Biology, Texas State University, San Marcos, TX, USA.

Nihal DharmasiriDepartment of Biology, Texas State University, San Marcos, TX, USA. nihal@txstate.edu.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Auxin, a major phytohormone, plays a pivotal role throughout the plant life cycle. While indole 3-acetic acid (IAA) is the major natural auxin found in plants, there are other natural and many synthetic chemicals with auxin activity. Ability to test the auxin activity in a simple cell-free assay will facilitate the identification of different chemicals with auxin activity. Moreover, the same assay can be used to identify the chemicals with antiauxin activity. Here we describe an in vitro pull-down assay that can be easily used to test auxin activity of different chemicals. Further, the concept of this assay can be used to test the activity of other hormones with similar mode of action or small ligand-based protein-protein interactions.

Indexed as

Biological AssayIndoleacetic AcidsPlant Growth RegulatorsProtein Interaction MappingArabidopsisArabidopsis ProteinsCell-Free SystemF-Box ProteinsProtein BindingReceptors, Cell SurfaceArabidopsis ProteinsF-Box Proteinsindoleacetic acidIndoleacetic AcidsPlant Growth RegulatorsReceptors, Cell SurfaceTIR1 protein, ArabidopsisAuxinCell-free assayGST-Aux/IAAJasmonic acidPull-downTIR1/AFBs

Identifiers

PMID41917670

DOI10.3390/ijms21041446

What OpenQuestion holds

Textmetadata

Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.