Evidence map›Paper›PMID 41917663›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2026

Single-Cell/Nucleus RNA-Sequencing for Phytohormone Signaling in Plants.

Neslihan Yaşar, Dilara Sedef Karagöz, Halis Batuhan Ünal, Ceyhun Kayıhan, Emre Aksoy

Abstract read
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Article in Methods in molecular biology (Clifton, N.J.), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Neslihan YaşarDepartment of Molecular Biology and Genetics, Faculty of Science and Letters, Başkent University, Ankara, Türkiye.ORCID https://orcid.org/0009-0003-0095-0807
Dilara Sedef KaragözDepartment of Molecular Biology and Genetics, Faculty of Science and Letters, Başkent University, Ankara, Türkiye.ORCID https://orcid.org/0009-0004-4332-0503
Halis Batuhan ÜnalDepartment of Molecular Biology and Genetics, Faculty of Science and Letters, Başkent University, Ankara, Türkiye.ORCID https://orcid.org/0000-0001-6901-4337
Ceyhun KayıhanDepartment of Molecular Biology and Genetics, Faculty of Science and Letters, Başkent University, Ankara, Türkiye. ckayihan@baskent.edu.tr.ORCID https://orcid.org/0000-0003-1684-4147
Emre AksoyDepartment of Biological Sciences, Faculty of Arts and Science, Middle East Technical University, Ankara, Türkiye.ORCID https://orcid.org/0000-0002-9410-2715

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

A class of organic, naturally occurring compounds known as phytohormones affects physiological functions in plants at trace levels. Phytohormones play important roles in plant growth, development, nutrient transport, and survival by regulating plant responses to abiotic stress and form the basis of ability of plants for adapting to changing conditions. To carry out some specific and distinct functions, cells of complex organisms implement gene regulation dynamics according to their cell types, including during the synthesis and control of various plant hormones. Rather than the traditional approaches for phytohormone quantification, single-cell RNA sequencing (scRNA-seq) and/or single-nucleus RNA sequencing (snRNA-seq) technologies that enable high-resolution mapping of cellular heterogeneity at the transcriptomic level, provide a novel approach for the phytohormone signaling. Here, we detailed the methodology of single cell/nucleus technologies to investigate underlying gene regulatory networks of plant hormone biology.

Indexed as

ArabidopsisCell NucleusPlant Growth RegulatorsSequence Analysis, RNASignal TransductionSingle-Cell AnalysisGene Expression Regulation, PlantRNA, PlantSingle-Cell Gene Expression AnalysisPlant Growth RegulatorsRNA, PlantArabidopsis thalianaNucleus isolationPhytohormonesProtoplast isolationSingle-cell RNA sequencing (scRNA-seq)Single-nucleus RNA sequencing (snRNA-seq)

Identifiers

PMID41917663

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.