Evidence map›Paper›PMID 41917463›Full record

ArticleNature biotechnology2026

Mirror-enhanced 4Pi-SMLM with one objective enables isotropic nanoscale imaging.

Zijing Yu, Bei Zheng, Yajing Zhan, Shuxin Li, Xulong Wang, Qiuyang Dai, Yanqin Chen, Zongfang Wei, Wenxuan Zhao, Linlin Chen and 7 more

Abstract read
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Article in Nature biotechnology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

17 authors.

Zijing Yu *Fudan University, Shanghai, China.ORCID http://orcid.org/0009-0005-1697-3872
Bei Zheng *State Key Laboratory of Gene Expression, School of Life Sciences, Westlake University, Hangzhou, China.
Yajing ZhanState Key Laboratory of Gene Expression, School of Life Sciences, Westlake University, Hangzhou, China.
Shuxin LiState Key Laboratory of Gene Expression, School of Life Sciences, Westlake University, Hangzhou, China.
Xulong WangState Key Laboratory of Gene Expression, School of Life Sciences, Westlake University, Hangzhou, China.
Qiuyang DaiState Key Laboratory of Gene Expression, School of Life Sciences, Westlake University, Hangzhou, China.
Yanqin ChenState Key Laboratory of Gene Expression, School of Life Sciences, Westlake University, Hangzhou, China.
Zongfang WeiState Key Laboratory of Gene Expression, School of Life Sciences, Westlake University, Hangzhou, China.
Wenxuan ZhaoState Key Laboratory of Gene Expression, School of Life Sciences, Westlake University, Hangzhou, China.
Linlin ChenState Key Laboratory of Gene Expression, School of Life Sciences, Westlake University, Hangzhou, China.
Jiaxin TangState Key Laboratory of Gene Expression, School of Life Sciences, Westlake University, Hangzhou, China.
Tianchang XiaState Key Laboratory of Gene Expression, School of Life Sciences, Westlake University, Hangzhou, China.
Lingjuan HeState Key Laboratory of Gene Expression, School of Life Sciences, Westlake University, Hangzhou, China.
Changliang LiuState Key Laboratory of Gene Expression, School of Life Sciences, Westlake University, Hangzhou, China.
Xudong WuState Key Laboratory of Gene Expression, School of Life Sciences, Westlake University, Hangzhou, China.
Xiaochun YuState Key Laboratory of Gene Expression, School of Life Sciences, Westlake University, Hangzhou, China.
Yongdeng ZhangState Key Laboratory of Gene Expression, School of Life Sciences, Westlake University, Hangzhou, China. zhangyongdeng@westlake.edu.cn.ORCID http://orcid.org/0000-0002-4569-8137

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Dual-objective 4Pi single-molecule localization microscopy (4Pi-SMLM) offers isotropic nanoscale resolution; however, its broader adoption is limited by instrumental complexity and stringent alignment requirements. Here we introduce mirror-enhanced 4Pi-SMLM (me4Pi-SMLM), a single-objective configuration that uses mirror-based retroreflection of the illumination beam to generate phase-tunable interference fringes. This design improves the axial resolution of astigmatism-based methods by approximately fivefold, delivering performance comparable to conventional 4Pi-SMLM while greatly reducing system complexity and maintenance. me4Pi-SMLM achieves near-isotropic localization precision of 2-3 nm in biological samples, enabling clear and unambiguous visualization of diverse ultrastructural features. Furthermore, it achieves sub-15 nm isotropic resolution in brain slices and facilitates high-fidelity two-colour imaging, nanoscale whole-cell reconstruction and live-cell imaging. me4Pi-SMLM can be seamlessly integrated into existing 3D-SMLM systems, enhancing performance with minimal cost and effort.

Identifiers

PMID41917463

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.