Evidence map›Paper›PMID 41917383›Full record

ArticleScientific reports2026

Comprehensive genomic identification of essential genes required for Brucella melitensis intracellular survival during macrophage infection.

Zhiqiang Jiang, Jiajun Gao, Mengda Liu, Xiangxiang Sun, Fei Qi, Weixing Shao, Wenlong Nan, Lu Chang, Kaihui Zhang, Xin Yan and 4 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

14 authors.

Zhiqiang Jiang *Professional Laboratory for Animal Brucellosis, Center of Zoonoses Surveillance, China Animal Health and Epidemiology Center, Qingdao, Shandong, China.
Jiajun Gao *Professional Laboratory for Animal Brucellosis, Center of Zoonoses Surveillance, China Animal Health and Epidemiology Center, Qingdao, Shandong, China.
Mengda LiuProfessional Laboratory for Animal Brucellosis, Center of Zoonoses Surveillance, China Animal Health and Epidemiology Center, Qingdao, Shandong, China.
Xiangxiang SunProfessional Laboratory for Animal Brucellosis, Center of Zoonoses Surveillance, China Animal Health and Epidemiology Center, Qingdao, Shandong, China.
Fei QiProfessional Laboratory for Animal Brucellosis, Center of Zoonoses Surveillance, China Animal Health and Epidemiology Center, Qingdao, Shandong, China.
Weixing ShaoProfessional Laboratory for Animal Brucellosis, Center of Zoonoses Surveillance, China Animal Health and Epidemiology Center, Qingdao, Shandong, China.
Wenlong NanProfessional Laboratory for Animal Brucellosis, Center of Zoonoses Surveillance, China Animal Health and Epidemiology Center, Qingdao, Shandong, China.
Lu ChangProfessional Laboratory for Animal Brucellosis, Center of Zoonoses Surveillance, China Animal Health and Epidemiology Center, Qingdao, Shandong, China.
Kaihui ZhangProfessional Laboratory for Animal Brucellosis, Center of Zoonoses Surveillance, China Animal Health and Epidemiology Center, Qingdao, Shandong, China.
Xin YanProfessional Laboratory for Animal Brucellosis, Center of Zoonoses Surveillance, China Animal Health and Epidemiology Center, Qingdao, Shandong, China.
Mingjun SunProfessional Laboratory for Animal Brucellosis, Center of Zoonoses Surveillance, China Animal Health and Epidemiology Center, Qingdao, Shandong, China.
Shufang SunProfessional Laboratory for Animal Brucellosis, Center of Zoonoses Surveillance, China Animal Health and Epidemiology Center, Qingdao, Shandong, China.
Xiaoxu FanProfessional Laboratory for Animal Brucellosis, Center of Zoonoses Surveillance, China Animal Health and Epidemiology Center, Qingdao, Shandong, China. fanxiaoxu@cahec.cn.
Haobo ZhangProfessional Laboratory for Animal Brucellosis, Center of Zoonoses Surveillance, China Animal Health and Epidemiology Center, Qingdao, Shandong, China. zhanghaobo@cahec.cn.

Funding

China Agriculture Research System CARS-36National Key Research and Development Program of China 2021YFD1800402
6 · The paper itself

Abstract

Brucella melitensis is a significant zoonotic pathogen transmissible via contact, digestive, and respiratory routes, posing severe threats to livestock economies and human health. While its virulence genes critically regulate intracellular survival and replication, the molecular mechanisms underlying pathogenesis remain elusive. This study employed Transposon Directed Insertion-site Sequencing (TraDIS) technology to identify key genes essential for the survival of Brucella melitensis strain 16 M within RAW 264.7 macrophages, resulting in the discovery of 374 anti-phagocytic associated essential genes. Through GO and KEGG analyses, these genes were categorized into eight functional classes, representative genes from each category were selected to generate respective gene-deletion mutants, with bacterial virulence and phenotypes validated via intracellular survival assays, in vitro stress tests, carbon source utilization experiments, and RT-qPCR. All ten constructed mutants exhibited reduced macrophage survival capabilities. Deletion of membrane-related genes cydDC and BME_RS07715 attenuated stress resistance, membrane stability, and immune evasion, knockout of metabolic gene ptsP impaired carbon source utilization and intracellular proliferation, and disruption of regulatory gene BME_RS00125 diminished TCA cycle activity and fatty acid metabolism, compromising energy supply and structural integrity. In this study, some key genes of Brucella surviving in macrophages were verified, and preliminarily elucidated the function of these genes in regulating bacterial virulence, providing a theoretical basis for exploring their mechanism.

Indexed as

Brucella melitensisBrucellosisGenes, BacterialGenes, EssentialMacrophagesAnimalsBacterial ProteinsGenomicsMiceMicrobial ViabilityRAW 264.7 CellsVirulenceBacterial ProteinsBrucella melitensisMacrophagesTraDISVirulence genes

Identifiers

PMID41917383
PMCPMC13187242

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.