ArticleMethods in molecular biology (Clifton, N.J.)2026
Microfluidic Shear-Based Assay for Assessing ADAMTS13 Activity Under Flow.
Article in Methods in molecular biology (Clifton, N.J.), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
A microfluidic system using the flow chamber techniques has long been utilized for thrombosis research. With the advent of technology, it has become possible to evaluate the relationship between blood flow dynamics and thrombus formation with greater precision. ADAMTS13, a plasma metalloprotease, efficiently cleaves von Willebrand factor (VWF) under flow conditions. The microfluidic shear-based assay is highly relevant for investigating the biological function of ADAMTS13. In this chapter, I describe the method using a microfluidic system to investigate the interaction between platelets and VWF, which is regulated by ADAMTS13. Whole blood samples obtained from mice with various genotypes, including wild type (WT), Adamts13-deficient (Adamts13
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