Evidence map›Paper›PMID 41917196›Full record

ArticleNature biomedical engineering2026

Programmable kinetic barcoding for multiplexed RNA detection with Cas13a.

Sungmin Son, Amy Lyden, Carlos F Ng, Andres Dextre, Jeffrey Shu, Stephanie I Stephens, Parinaz Fozouni, Gavin J Knott, Dylan C J Smock, Tina Y Liu and 6 more

Abstract read
PubMed Publisher
In one paragraph

Article in Nature biomedical engineering, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Article
  2. Article
  3. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

16 authors.

Sungmin SonDepartment of Bioengineering, University of California, Berkeley, CA, USA. smson@kaist.ac.kr.ORCID http://orcid.org/0000-0001-7481-4056
Amy LydenDepartment of Bioengineering, University of California, Berkeley, CA, USA.ORCID http://orcid.org/0000-0002-5909-9995
Carlos F NgDepartment of Bioengineering, University of California, Berkeley, CA, USA.
Andres DextreDepartment of Bioengineering, University of California, Berkeley, CA, USA.ORCID http://orcid.org/0009-0007-8292-2816
Jeffrey ShuGladstone Infectious Disease Institute, Gladstone Institutes, San Francisco, CA, USA.ORCID http://orcid.org/0000-0002-9174-9302
Stephanie I StephensGladstone Infectious Disease Institute, Gladstone Institutes, San Francisco, CA, USA.
Parinaz FozouniGladstone Infectious Disease Institute, Gladstone Institutes, San Francisco, CA, USA.
Gavin J KnottDepartment of Molecular and Cell Biology, University of California, Berkeley, CA, USA.ORCID http://orcid.org/0000-0002-9007-6273
Dylan C J SmockDepartment of Molecular and Cell Biology, University of California, Berkeley, CA, USA.
Tina Y LiuDepartment of Molecular and Cell Biology, University of California, Berkeley, CA, USA.ORCID http://orcid.org/0000-0003-1297-7102
Daniela BoehmGladstone Infectious Disease Institute, Gladstone Institutes, San Francisco, CA, USA.
Camille SimoneauGladstone Infectious Disease Institute, Gladstone Institutes, San Francisco, CA, USA.
G Renuka KumarGladstone Infectious Disease Institute, Gladstone Institutes, San Francisco, CA, USA.
Jennifer A DoudnaGladstone Infectious Disease Institute, Gladstone Institutes, San Francisco, CA, USA.ORCID http://orcid.org/0000-0001-9161-999X
Melanie OttGladstone Infectious Disease Institute, Gladstone Institutes, San Francisco, CA, USA. melanie.ott@gladstone.ucsf.edu.ORCID http://orcid.org/0000-0002-5697-1274
Daniel A FletcherDepartment of Bioengineering, University of California, Berkeley, CA, USA. fletch@berkeley.edu.ORCID http://orcid.org/0000-0002-1890-5364

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Rapid identification of viral infections and specific variants in patient samples requires a simple and multiplexed RNA detection method that does not rely on DNA sequencing. Although recent direct detection assays based on CRISPR-Cas13a offer rapid RNA detection by avoiding reverse transcription and DNA amplification required of gold-standard PCR assays, these assays are not easily multiplexed to detect multiple viruses or variants without dividing the sample into separate reactions. Here we show that Cas13a acting on single-target RNAs exhibits variable nuclease activity that depends on the interaction between the target RNA and crRNA. To exploit this feature for multiplexed detection, we devised a crRNA modification strategy that enables programmable tuning of Cas13a's nuclease enzymatic rates. Using a droplet-based Cas13a assay, we demonstrate that kinetic signatures can be harnessed to differentiate among respiratory viruses and SARS-CoV-2 variants in contrived and clinical samples. This kinetic barcoding strategy can be extended to additional RNA targets through simple modification of crRNAs.

Identifiers

PMID41917196

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.