Evidence map›Paper›PMID 41917157›Full record

ArticleNpj imaging2026

Maximizing photon utilization in spectroscopic single-molecule localization microscopy using symmetrically dispersed dual-wedge prisms.

Wei-Hong Yeo, Benjamin Brenner, Menglin Shi, Youngseop Lee, Junghun Kweon, Cheng Sun, Hao F Zhang

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Npj imaging, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

7 authors.

Wei-Hong YeoDepartment of Biomedical Engineering, Northwestern University, Evanston, IL, USA.
Benjamin BrennerDepartment of Biomedical Engineering, Northwestern University, Evanston, IL, USA.
Menglin ShiDepartment of Biomedical Engineering, Northwestern University, Evanston, IL, USA.
Youngseop LeeDepartment of Biomedical Engineering, Northwestern University, Evanston, IL, USA.
Junghun KweonDepartment of Biomedical Engineering, Northwestern University, Evanston, IL, USA.
Cheng SunDepartment of Mechanical Engineering, Northwestern University, Evanston, IL, USA.
Hao F ZhangDepartment of Biomedical Engineering, Northwestern University, Evanston, IL, USA. hfzhang@northwestern.edu.

Funding

NIH HHS R01GM139151, R01GM140478, U54CA268084, and R01GM143397Northwestern University Christina Enroth-Cugell Graduate Research Award
6 · The paper itself

Abstract

Single-molecule localization microscopy (SMLM) achieves super-resolution by analyzing individual fluorescence emissions. Spectroscopic SMLM (sSMLM) extends this by capturing spectral data but suffers from reduced precision due to photon splitting. We present a symmetrically dispersed dual-wedge prism (SDDWP)-sSMLM system that maximizes photon use for both spatial and spectral analyses. Fluorescence photons are equally split and symmetrically dispersed using two identical dual-wedge prisms, with spatial positions and spectra computationally extracted using all collected photons. This approach improves spatial and spectral precisions by 27% and 48%, respectively, over prior sSMLM systems. Using a single excitation laser, we achieve multiplexed imaging of peroxisomes, microtubules, and mitochondria labeled with spectrally overlapping dyes (DY-634, AF647, CF660C). We also demonstrate massively parallel tracking of spectrally tagged nanoparticles at concentrations five times higher than previously reported. The entire system is integrated into a compact module, facilitating upgrade from an existing SMLM system to a spectroscopic SMLM.

Identifiers

PMID41917157
PMCPMC13039816

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.