Evidence map›Paper›PMID 41916304›Full record

ArticleCell reports methods2026

Expanded applications of bioluminescence microscopy with phasor analysis.

Lila P Halbers, Caroline K Brennan, Lorenzo Scipioni, Giulia Tedeschi, Zachary R Torrey, Kshitij Parag-Sharma, Bryan Labra, Christoph Gohlke, Antonio L Amelio, Michelle A Digman and 1 more

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In one paragraph

Article in Cell reports methods, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Lila P HalbersDepartment of Pharmaceutical Sciences, University of California, Irvine, Irvine, CA, USA.
Caroline K BrennanDepartment of Chemistry, University of California, Irvine, Irvine, CA, USA.
Lorenzo ScipioniLaboratory for Fluorescence Dynamics, Department of Biomedical Engineering, University of California, Irvine, Irvine, CA, USA.
Giulia TedeschiLaboratory for Fluorescence Dynamics, Department of Biomedical Engineering, University of California, Irvine, Irvine, CA, USA.
Zachary R TorreyDepartment of Chemistry, University of California, Irvine, Irvine, CA, USA.
Kshitij Parag-SharmaGraduate Curriculum in Cell Biology & Physiology, Biological & Biomedical Sciences Program, UNC School of Medicine, The University of North Carolina at Chapel Hill, Chapel Hill, NC, USA.
Bryan LabraLineberger Comprehensive Cancer Center, The University of North Carolina at Chapel Hill, Chapel Hill, NC, USA.
Christoph GohlkeLaboratory for Fluorescence Dynamics, Department of Biomedical Engineering, University of California, Irvine, Irvine, CA, USA.
Antonio L AmelioLineberger Comprehensive Cancer Center, The University of North Carolina at Chapel Hill, Chapel Hill, NC, USA; Department of Otolaryngology/Head and Neck Surgery, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA; Department of Tumor Microenvironment and Metastasis, H. Lee Moffitt Cancer Center & Research Institute, Tampa, FL, USA; Department of Head and Neck-Endocrine Oncology, H. Lee Moffitt Cancer Center & Research Institute, Tampa, FL, USA.
Michelle A DigmanLaboratory for Fluorescence Dynamics, Department of Biomedical Engineering, University of California, Irvine, Irvine, CA, USA. Electronic address: mdigman@uci.edu.
Jennifer A PrescherDepartment of Pharmaceutical Sciences, University of California, Irvine, Irvine, CA, USA; Department of Chemistry, University of California, Irvine, Irvine, CA, USA; Department of Molecular Biology and Biochemistry, University of California, Irvine, Irvine, CA, USA. Electronic address: jpresche@uci.edu.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Bioluminescence is a powerful technique for monitoring biological processes in vitro and in vivo. A variety of bioluminescent reporters have been developed that span the visible spectrum, but simultaneous detection remains challenging due to substantial spectral overlap. To address this issue, we previously developed bioluminescent phasor analysis to resolve spectrally similar probes. In the current work, we characterize the phasor outputs of several common luciferase reporters and expand the method for distinguishing complex mixtures in tissues. We further apply bioluminescent phasor for imaging biosensors in multidimensional models. Subtle changes in bioluminescent outputs were readily resolved, enabling previously indistinguishable changes in analyte-dependent spectra to be detected. Collectively, this work broadens the utility of bioluminescent phasor imaging for multiplexed imaging applications.

Indexed as

Luminescent MeasurementsMicroscopyAnimalsBiosensing TechniquesHumansLuciferasesLuciferasesbioluminescencebiosensorCP: imagingimagingluciferaseluciferinspectral phasor

Identifiers

PMID41916304
PMCPMC13106971

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.