ArticleCell reports methods2026
Microfluidics-enabled proteomic profiling reveal iron-driven immune evasion by an antimicrobial-resistant pathogen.
Article in Cell reports methods, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
Dissecting host-pathogen interactions is challenging due to heterogeneous co-cultures and limited separation methods. Here, we developed a label-free microfluidic chip enabling reproducible separation of Klebsiella pneumoniae and murine macrophages during co-culture for high-resolution proteomic analysis. Using an optimized 1.4 μm filter, the platform preserved cell viability while improving host protein identification and enriching immune-associated proteins compared to traditional scraping and supernatant collection. Chip-isolated non-phagocytosed bacteria displayed distinct proteome profiles, including reduced metabolic enzymes and increased biosynthetic and iron-binding proteins. Iron-associated proteins were uniquely enriched in this population, and functional assays confirmed that iron promotes macrophage evasion and bacterial survival. Together, these results establish a microfluidic-proteomic workflow for resolving complex host-pathogen dynamics and propose an iron-dependent mechanism of immune evasion. This approach reduces sample handling and cross-contamination while preserving cellular structure, providing a powerful framework for studying infection biology and identifying therapeutic targets.
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