ArticleAdvanced science (Weinheim, Baden-Wurttemberg, Germany)2026
Polymer Matrix-Based 3D Culture Significantly Enhances the Differentiation and Immunomodulatory Functions of Human Adipose-Derived Stem Cells.
Article in Advanced science (Weinheim, Baden-Wurttemberg, Germany), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
1 citing paper in PubMed.
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Authors and funding
7 authors.
Funding
Abstract
Human adipose-derived stem cells (hADSCs) possess immunomodulatory and multipotent properties, making them attractive candidates for regenerative therapies. However, conventional tissue culture plate (TCP)-based 2D adherent cultures or widely used ultra-low attachment (ULA) spheroid systems often compromise differentiation potential and therapeutic efficacy of hADSCs. Here, we report a synthetic polymer matrix, poly-Z, that enables the formation of hADSC spheroids with significantly enhanced biological functions. Compared to ULA or TCP culture systems, poly-Z-cultured hADSC spheroids exhibited improved cell viability, enriched extracellular matrix deposition, elevated pluripotency marker expression, and superior tri-lineage differentiation capacity. Notably, poly-Z spheroids displayed a distinct immunomodulatory signature characterized by significantly elevated secretion of indoleamine-pyrrole 2,3-dioxygenase (IDO) and prostaglandin E2 (PGE2), and showed enhanced in vivo persistence after transplantation. In mouse models of acute colitis and acetaminophen-induced liver injury, poly-Z-cultured hADSC spheroids markedly alleviated inflammation, reduced tissue damage, and modulated pro- and anti-inflammatory cytokine levels more effectively than ULA or TCP-derived cells. These findings establish the poly-Z matrix as a potent 3D culture platform that enhances the functional and therapeutic potential of hADSCs for clinical applications.
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