Protocol for differentiation and efficient AAV-mediated gene delivery to hiPSC-derived microglia for functional studies.

Christina N Heiss, Anastasia Mihai, Stefanie Fruhwürth, Andreas Björefeldt

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In one paragraph

Article in STAR protocols, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0cells of the map it votes in

0citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Christina N HeissDepartment of Psychiatry and Neurochemistry, Institute of Neuroscience and Physiology, University of Gothenburg, Gothenburg, Sweden.

Anastasia MihaiDepartment of Physiology, Institute of Neuroscience and Physiology, University of Gothenburg, Gothenburg, Sweden.

Stefanie FruhwürthDepartment of Psychiatry and Neurochemistry, Institute of Neuroscience and Physiology, University of Gothenburg, Gothenburg, Sweden. Electronic address: stefanie.fruhwurth@gu.se.

Andreas BjörefeldtDepartment of Physiology, Institute of Neuroscience and Physiology, University of Gothenburg, Gothenburg, Sweden. Electronic address: andreas.bjorefeldt@gu.se.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Human induced pluripotent stem cell (hiPSC)-derived microglia (hiMG) constitute a powerful platform to study human microglial biology in health and disease. Here, we present a protocol for microglial differentiation and efficient gene delivery to these cells using adeno-associated virus (AAV). We describe steps for differentiation, viral transduction, and validation using flow cytometry and fluorescence imaging. Finally, to demonstrate the utility of this approach, we outline procedures to record and analyze calcium activity in hiMG using the genetically encoded sensor GCaMP8s. For complete details on the use and execution of this protocol, please refer to Fruhwürth et al.

Indexed as

Cell DifferentiationDependovirusGene Transfer TechniquesInduced Pluripotent Stem CellsMicrogliaGenetic VectorsHumansTransduction, GeneticNeuroscienceStem CellsTissue Engineering

Identifiers

PMID41915476

DOI10.1016/j.xpro.2026.104455

PMCPMC13068558

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.