ArticleMolecular biology reports2026
m6A RNA modifications in neurosystem genes as early markers for osteoporosis with iron accumulation.
Article in Molecular biology reports, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
0 citing papers in PubMed.
No citing paper in PubMed yet.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors.
Funding
Abstract
backgroundPostmenopausal osteoporosis is a prevalent bone disorder, and recent evidence suggests that iron accumulation, particularly within the nervous system, may exacerbate bone loss through epigenetic mechanisms. This study aims to investigate the role of m6A RNA methylation in the regulation of bone metabolism under conditions of iron overload in a postmenopausal osteoporotic model.
methodsFifteen female C57BL/6J mice were divided into three groups: sham-operated, ovariectomized (ovx), and ovariectomized with iron–dextran intervention (ovx+iron). Ovariectomy was performed at 8 weeks of age, followed by 8 weeks of iron–dextran administration to establish the model. Bone RNA samples were subjected to methylated RNA immunoprecipitation sequencing (MeRIP-seq) to profile m6A modifications. Bone morphology and iron status were evaluated using microCT scanning, immunohistochemistry, and Prussian blue staining.
resultsThe ovx+iron group exhibited a higher number of m6A peaks (27,167) compared to the ovx group (21,441), with significant differences in methylation patterns. Upregulated differentially methylated genes (DMGs) were enriched in pathways related to nervous system development and skeletal morphogenesis, while downregulated DMGs were associated with epithelial morphogenesis and Wnt signaling. Integrative analysis identified 150 genes with concurrent alterations in mRNA expression and m6A modification, highlighting neural regulatory influences on bone mass under iron accumulation. Notably, Lep, a critical neural regulator, showed reduced expression and decreased m6A methylation in the ovx+iron group, which was further confirmed at the protein level by immunohistochemistry.
conclusionIron accumulation induces significant changes in m6A methylation, which may disrupt bone metabolism by modulating neural factors such as Leptin. These findings suggest that Leptin could serve as a potential early diagnostic biomarker for postmenopausal osteoporosis complicated by iron overload.
Indexed as
Identifiers
41915261What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.