ArticleNanotechnology, science and applications2026
Labeling of Amine-Modified Material Surfaces with PFB-Fluorescein.
Article in Nanotechnology, science and applications, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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11 authors.
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Abstract
Introduction: Fluorescent labeling is a widely used method for visualizing and tracking materials. 5-(Pentafluorobenzoylamino) fluorescein (PFB-F) is a dye in which the pentafluorobenzoylamino group substituent functions as a reactive linker. While PFB-F has been used for labeling biomolecules, its application in labeling surfaces has not been explored yet. Here, we report straightforward method for attaching PFB-F to amine-modified material surfaces requiring only dimethyl sulfoxide (DMSO) solvent and room temperature (RT). Methods: We conducted nuclear magnetic resonance (NMR) and thin layer chromatography (TLC) analysis on a mock reaction involving PFB-F and Results: NMR and TLC analysis confirmed covalent bonding between PFB-F and primary amines via nucleophilic aromatic substitution (S Discussion: These results demonstrate that PFB-F is a versatile and effective reagent for stable surface labeling. This study paves the way for broader applications of PFB-F in material design.
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