Evidence map›Paper›PMID 41913290›Full record

ArticleGenome biology2026

A H3K27me3 reader complex couples H3K27me3 accumulation to nascent transcription of transposable elements in Paramecium.

Thomas Balan, Ambre Petitalot, Julien Richard Albert, Louise Abbou, Guillaume Chevreux, Bassem Al-Sady, Bihter Özdemir Aygenli, Till Bartke, Raphaël Margueron, Sandra Duharcourt

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In one paragraph

Article in Genome biology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Thomas BalanUniversité Paris Cité, CNRS, Institut Jacques Monod, 75013, Paris, France.
Ambre PetitalotInstitut Curie, Paris Sciences Et Lettres Research University, INSERM, CNRS, 75005, Paris, France.
Julien Richard AlbertUniversité Paris Cité, CNRS, Institut Jacques Monod, 75013, Paris, France.
Louise AbbouUniversité Paris Cité, CNRS, Institut Jacques Monod, 75013, Paris, France.
Guillaume ChevreuxUniversité Paris Cité, CNRS, Institut Jacques Monod, 75013, Paris, France.
Bassem Al-SadyDepartment of Microbiology & Immunology, George Williams Hooper Foundation, University of California San Francisco, San Francisco, CA, USA.
Bihter Özdemir AygenliInstitute of Functional Epigenetics, Helmholtz Zentrum München, 85764, Neuherberg, Germany.
Till BartkeInstitute of Functional Epigenetics, Helmholtz Zentrum München, 85764, Neuherberg, Germany.
Raphaël MargueronInstitut Curie, Paris Sciences Et Lettres Research University, INSERM, CNRS, 75005, Paris, France.
Sandra DuharcourtUniversité Paris Cité, CNRS, Institut Jacques Monod, 75013, Paris, France. sandra.duharcourt@ijm.fr.

Funding

Agence Nationale de la Recherche project "MODIFICATION" ANR-25-CE12-7757Agence Nationale pour la Recherche project "POLYCHROME" ANR-19-CE12-0015Agence Nationale pour la Recherche project "SELECTION" ANR-23-CE12-0027Deutsche Forschungsgemeinschaft DFG Project ID 431163844EUR G.E.N.E. Graduate School ANR 17 EURE 0013Fondation pour la Recherche Médicale Equipe FRM EQU202203014643Fondation pour la Recherche Médicale FDT202404018139LABEX Who Am I? ANR-11- LABX-0071; ANR-11-IDEX-0005-02
6 · The paper itself

Abstract

backgroundThe ability to deposit histone H3K27-trimethyl (me3) marks is essential for transcriptional repression by Polycomb Repressive Complex 2 (PRC2). This is largely attributed to Polycomb repressive complex 1 (PRC1), whose recruitment is H3K27me3-dependent. Yet it is unclear how H3K27me3 contributes to transcription regulation independently of PRC1.

resultsTo address this question, we identified H3K27me3-binding proteins in the unicellular eukaryote Paramecium where PRC2 targets transposable elements (TEs) and PRC1 is absent. We show that the chromodomain protein Firefly is a H3K27me3 reader in vitro and accumulates onto TEs in a H3K27me3-dependent manner. We also identify Firefly interactors: Sleepy, a coiled-coil containing protein and TfIIs4, a transcription elongation factor. We show that Firefly, Sleepy and TfIIs4 are jointly required in vivo for correct H3K27me3 accumulation and nascent transcription at TEs.

conclusionsThis positive feedback loop to enrich H3K27me3 at TEs is analogous to self-reinforcing loops leading to H3K9 methylation at repeats in fungi, plants and animals. Our work reveals a unique association between H3K27me3 readers and active transcription.

Indexed as

DNA Transposable ElementsHistonesParameciumProtozoan ProteinsTranscription, GeneticMethylationParamecium tetraureliaDNA Transposable ElementsHistonesProtozoan ProteinsChromodomainCiliatesHistone modificationsNascent transcriptionPolycomb repressive complexProgrammed DNA eliminationTransposable elements

Identifiers

PMID41913290
PMCPMC13159322

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.