ArticleNeurogastroenterology and motility2026
Acacetin Alleviates Loperamide-Induced Functional Constipation by Inhibiting P53-Mediated Apoptosis in Colonic Epithelial Cells.
Article in Neurogastroenterology and motility, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
0 citing papers in PubMed.
No citing paper in PubMed yet.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
10 authors.
Funding
Abstract
backgroundFunctional constipation (FC) is a prevalent gastrointestinal disorder with limited therapeutic options. Yunweiling (YWL), a traditional Chinese herbal medicine from Radix Inulae extract, has been used for treating constipation, but its underlying mechanisms remain unclear. This study aimed to investigate the protective effects and mechanisms of acacetin, a bioactive compound of YWL, in alleviating FC.
methodsMolecular docking analysis was performed to identify the targets of bioactive compounds in YWL formula. A loperamide-induced FC mouse model was established, and the effects of acacetin on gastrointestinal motility, colon histology, and signaling pathways were evaluated. Human colonic mucosal epithelial cells were used to investigate the protective effects of YWL extracts and acacetin against loperamide-induced apoptosis and the involvement of the tumor protein P53 (P53) signaling pathway.
resultsMolecular docking analysis revealed acacetin as a bioactive chemical targeting P53 protein. In the FC mouse model, acacetin administration improved gastrointestinal motility, alleviated colon mucosal damage, and suppressed the upregulation of P53 and its downstream apoptotic signaling. In colonic epithelial cells, loperamide induced apoptosis by activating P53 and inhibiting Akt1 signaling, which was attenuated by YWL extract and acacetin treatment. The protective effects of YWL and acacetin were abrogated by the P53 activator Nutlin-3a, suggesting that the inhibition of P53 signaling is crucial for their anti-apoptotic properties.
conclusionAcacetin alleviates loperamide-induced FC by inhibiting P53-mediated apoptosis in colonic epithelial cells, providing new insights into the therapeutic mechanisms of traditional Chinese herbal medicine YWL in treating constipation.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.