ArticleEquine veterinary journal2026
Mass culture of equine synovial fluid-derived mesenchymal stromal cells using nonwoven polyethylene terephthalate fabrics.
Article in Equine veterinary journal, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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6 authors.
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Abstract
backgroundCulture protocols need to yield 100 million equine synovial fluid (SF)-derived mesenchymal stromal cells (SF-MSCs) in around 3 weeks are needed, before these cells can be evaluated as agents of articular repair in clinical trials.
objectivesTo investigate mass culture of equine SF-MSC culture protocols using nonwoven polyethylene terephthalate (PET) fabrics for the potential to meet the targets for clinical trials. STUDY
designIn vitro experiments.
methodsSF samples were collected from the carpal joints in thoroughbred racehorses (n = 21) undergoing arthroscopic surgery and cultured in 10% fetal bovine serum (FBS-protocol; n = 7) or 10% equine serum (ES-protocol; n = 7)-supplemented medium. Primary (P0) SF-MSCs were isolated by dish culture and then passaged on nonwoven PET fabrics in culture bottles at a seeding density of 1 × 10
resultsUnder the FBS and ES protocols, 0.84 ± 0.82 × 10 MAIN LIMITATIONS: Visualisation of SF-MSC attached to PET nonwoven fabric, counting of SF-MSCs attached to and detached from PET nonwoven fabric, and daily quantification of SF-MSC proliferating on fabrics.
conclusionsTo support progress to clinical trials, of a sufficient number (100 million) of equine SF-MSCs need to be harvested in around 3 weeks. These goals were achieved with the FBS protocol, but further optimisation is needed for the ES protocol. Our findings on in vitro MSCs amplification suggest that SF, a potential source for non-invasively collected MSCs, is available as a promising matrix for therapeutic strategies using autologous transplantation.
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