Evidence map›Paper›PMID 41912816›Full record

ArticleDoklady biological sciences : proceedings of the Academy of Sciences of the USSR, Biological sciences sections2026

Comprehensive Optimization of Agroinfiltration in Transient Expression System in Nicotiana tabacum with RUBY Visible Reporter.

M R Sorokina, Yu A Yugay, A I Degtyarenko, A A Sadykov, Yu N Shkryl, V P Bulgakov

Abstract read
PubMed Publisher
In one paragraph

Article in Doklady biological sciences : proceedings of the Academy of Sciences of the USSR, Biological sciences sections, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

M R SorokinaFederal Scientific Center of the East Asia Terrestrial Biodiversity, Far Eastern Branch, Russian Academy of Sciences, Vladivostok, Russia.
Yu A YugayFederal Scientific Center of the East Asia Terrestrial Biodiversity, Far Eastern Branch, Russian Academy of Sciences, Vladivostok, Russia.
A I DegtyarenkoFederal Scientific Center of the East Asia Terrestrial Biodiversity, Far Eastern Branch, Russian Academy of Sciences, Vladivostok, Russia.
A A SadykovFederal Scientific Center of the East Asia Terrestrial Biodiversity, Far Eastern Branch, Russian Academy of Sciences, Vladivostok, Russia.
Yu N ShkrylFederal Scientific Center of the East Asia Terrestrial Biodiversity, Far Eastern Branch, Russian Academy of Sciences, Vladivostok, Russia. yn80@mail.ru.
V P BulgakovFederal Scientific Center of the East Asia Terrestrial Biodiversity, Far Eastern Branch, Russian Academy of Sciences, Vladivostok, Russia.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Agrobacterium-mediated transient expression is one of the most popular tools in functional genomics and plant biotechnology. However, its efficiency and reproducibility depend on multiple factors. Agroinfiltration conditions were optimized comprehensively to enhance the expression level of betalain biosynthetic genes in the model plant Nicotiana tabacum with the use of the RUBY visual reporter system. The effects of bacterial suspension concentration, infiltration buffer composition, and delivery method were evaluated. The highest expression efficiency was achieved with a suspension of OD

Indexed as

AgrobacteriumNicotianaPlant ProteinsPlants, Genetically ModifiedBetalainsGene Expression Regulation, PlantGenes, ReporterBetalainsPlant Proteinsagrobacterium-mediated transient expressionbetalainNicotiana tabacumRUBY

Identifiers

PMID41912816

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.