ArticleWorld journal of microbiology & biotechnology2026
A potassium titanate whisker-assisted method for genetic transformation of Phanerochaete chrysosporium using a Cas9-sGFP expression vector.
Article in World journal of microbiology & biotechnology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
0 citing papers in PubMed.
No citing paper in PubMed yet.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Phanerochaete chrysosporium, a model white-rot fungus, plays a central role in lignin degradation but has remained recalcitrant to genetic manipulation due to inherently low transformation efficiencies. In this study, we present a novel whisker-assisted transformation method employing potassium titanate whiskers combined with freeze–thaw pretreatment, L-ornithine supplementation, and sonication to enable efficient DNA delivery into P. chrysosporium. Using the Cas9-sGFP construct as a reporter system, transformants were selected on hygromycin and validated through PCR and fluorescence microscopy. Our protocol consistently generated hygromycin-resistant transformants, achieving a reproducible transformation efficiency of ~ 0.26%, outperforming Agrobacterium-mediated transformation and comparable to shockwave-based approaches. The successful integration and functional expression of the sGFP reporter confirmed the stability and reliability of this approach. This study provides the first evidence of whisker-mediated delivery and stable integration of exogenous DNA into the genomic DNA of a white-rot fungus. By providing a simple, cost-effective, and reproducible transformation strategy, this work addresses a longstanding bottleneck in fungal biotechnology. The method provides a scalable alternative for CRISPR-based genome engineering in ligninolytic fungi and unlocks opportunities for metabolic engineering of P. chrysosporium, including targeted gene disruption, overexpression of ligninolytic enzymes, and expression of heterologous biosynthetic pathways for the sustainable production of industrially valuable compounds.
Indexed as
Identifiers
41912740What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.