ArticleMicrosystems & nanoengineering2026
Design of an automated cell batch microinjection system based on magnetic tweezers for zebrafish embryos.
Article in Microsystems & nanoengineering, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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9 authors.
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Abstract
Batch microinjection significantly enhances throughput and reproducibility in gene delivery and developmental studies, thereby accelerating the advancement of intelligent experimentation in the life sciences. In this work, we propose a novel visual-guided automated batch microinjection system based on magnetic tweezers, designed for zebrafish embryos. The system enables rapid and precise cell reorientation and puncture by integrating a microfluidic chip with coupled fluidic and magnetic actuation for cell manipulation. To address the challenge of robust perception in a narrow microscopic field, we introduce a microscopic manipulation perception network (MMPN), which incorporates a dual-backbone architecture and an attention mechanism to enhance feature extraction and recognition accuracy. Experimental validation demonstrates a detection mean average precision (mAP) of 98.8% and a segmentation accuracy of 98.4%. The proposed system achieves an average operation time of 33.8 seconds per cell, with a cell survival rate of 88% and a reorientation error as low as 2.1
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