ReviewMicroscopy (Oxford, England)2026
Diverse applications of volume CLEM analysis in cell biology: brief overview, unique techniques and recent notable works.
Review in Microscopy (Oxford, England), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
The technology of volume electron microscopy (vEM), which enables three-dimensional (3D) observation of organelles, cells and even tissues at the nanoscale, is advancing and being applied to various fields of life science. As the demand for vEM grows, there is an increasing need for Volume Correlative Light and Electron Microscopy (Volume CLEM) to capture targets without missing the region of interest (ROI) or to observe the localization of molecules (often proteins) at the exact location of the fluorescent signals. This mini review provides an overview of the current state of Volume CLEM and introduces several unique approaches. My aim is to share the diverse fundamental technologies and broad application scope of this technique. vEM provides nanoscale imaging for life science applications. CLEM, is very powerful for linking molecular localization to ultrastructure. This mini review outlines advances, distinctive approaches and its broad technological and application scope.
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