ArticleJournal of veterinary internal medicine2026
Molecular and serological evidence of Nairobi sheep disease virus in Haemaphysalis longicornis and sheep in eastern Shandong Peninsula, China.
Article in Journal of veterinary internal medicine, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
0 citing papers in PubMed.
No citing paper in PubMed yet.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
11 authors.
Funding
Abstract
backgroundNairobi sheep disease virus (NSDV), a highly pathogenic tick-borne orthonairovirus affecting ruminants, represents an emerging zoonotic threat. While endemic to East Africa, recent reports confirm its presence in Asia. HYPOTHESIS/
objectivesThis study investigated NSDV circulation in the eastern Shandong Peninsula. ANIMALS: A total of 745 Haemaphysalis longicornis ticks (grouped into 139 pools) and 246 ruminant serum samples were collected during 2021 field surveillance in Weifang and Yantai regions.
methodsMolecular detection employed quantitative reverse transcription PCR and nested PCR assays, with genomic characterization through complete and partial segment sequencing. Serological analysis used ELISA and immunofluorescence assays (IFAs). Phylogenetic reconstruction was performed using maximum-likelihood methods.
resultsNairobi sheep disease virus RNA was detected in 9 tick pools (minimum infection rate: 1.2%). Genomic analysis revealed one complete genome (L: 11 999 bp; M: 5013 bp; and S: 1469 bp) and 4 partial S segment sequences (975 bp) showing > 98% nucleotide identity with Chinese reference strains and 100% identity among themselves. Phylogenetically, Shandong isolates clustered with other Chinese NSDV variants, indicating local evolutionary adaptation. Seroprevalence reached 5.7% (14/246) among ruminants, suggesting local virus exposure. CONCLUSIONS AND CLINICAL IMPORTANCE: This molecular and serological evidence of NSDV in eastern Shandong suggests the potential presence of the virus beyond its historical range. The close genetic relationship with other Chinese strains suggests regional spread rather than independent introductions. These findings underscore the need for enhanced surveillance in tick-ruminant systems and the development of control strategies to mitigate economic and zoonotic risks.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.