Evidence map›Paper›PMID 41910171›Full record

ArticleVeterinary medicine and science2026

Bovine Tuberculosis in Raw Milk: A Herd-Level Prevalence Study by PCR and ELISA Testing in Sylhet Region of Bangladesh.

Lipi Rani Basak, Md-Tariqul Islam, Sabuj Kanti Mazumder, Dewan Mohammad Mahbubur Rajib, Mohammad Nizam Uddin Chowdhury, Md Irtija Ahsan, Md Mukter Hossain, Sultan Ahmed, A T M Mahbub-E-Elahi

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Article in Veterinary medicine and science, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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4 · The record

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5 · Who and what money

Authors and funding

9 authors.

Lipi Rani BasakDepartment of Livestock Services, Bangladesh.
Md-Tariqul IslamDepartment of Microbiology and Immunology, Sylhet Agricultural University, Sylhet, Bangladesh.ORCID https://orcid.org/0000-0002-6760-3362
Sabuj Kanti MazumderDepartment of Genetics and Fish Breeding, Gazipur Agricultural University, Gazipur, Bangladesh.ORCID https://orcid.org/0000-0002-3604-2363
Dewan Mohammad Mahbubur RajibDepartment of Microbiology and Immunology, Sylhet Agricultural University, Sylhet, Bangladesh.
Mohammad Nizam Uddin ChowdhuryBangladesh Forest Department, Wildlife Center, Gazipur, Bangladesh.
Md Irtija AhsanDepartment of Epidemiology and Public Health, Sylhet Agricultural University, Sylhet, Bangladesh.ORCID https://orcid.org/0000-0002-1761-2367
Md Mukter HossainDepartment of Medicine, Sylhet Agricultural University, Sylhet, Bangladesh.ORCID https://orcid.org/0000-0002-8506-1202
Sultan AhmedDepartment of Microbiology and Immunology, Sylhet Agricultural University, Sylhet, Bangladesh.
A T M Mahbub-E-ElahiDepartment of Microbiology and Immunology, Sylhet Agricultural University, Sylhet, Bangladesh.

Funding

Ministry of Science and Technology fellowship, Government of the People's Republic of Bangladesh
6 · The paper itself

Abstract

introductionBovine tuberculosis (bTB) has been a major cause of morbidity and mortality in farm animals and the recent surge of Mycobacterium tuberculosis complex (MTC) in developing countries poses a serious threat to public and animal health. This study aimed to assess the herd-level prevalence and associated risk factors of bovine TB in cattle in northeast regions of Bangladesh.

methodsA total of 485 dairy samples (385 from dairy milk and 100 from vendor's milk) were tested by Polymerase Chain Reaction (PCR) and indirect ELISA (enzyme-linked immunosorbent assay) technique at 16 Upazilas from 4 districts in the Sylhet division of Bangladesh. Genomic DNA extracted from milk samples were targeted at IS6110 and RV1506c genomic fragments for PCR.

resultsAmong the 385 milk samples tested, 15 milk samples were positive for Mycobacterium genus by PCR (3.90%, 95% CI: 1.95-5.84). Also, 10 samples were found to be positive for M. bovis and the prevalence was 2.60% (95% CI: 1.00-4.19) and only 2 milk samples were positive for M. tuberculosis by PCR whose prevalence was 0.52% (95% CI: 0.00-1.24) respectively in individual milk samples. In vendor's milk sample, the trend was lowered for each bacterium and indirect ELISA results agreed with a similar pattern of prevalence. Cows having chronic cough was one of the significant risk factors of herd-level prevalence.

conclusionFindings from this study necessitate a comprehensive program for TB surveillance of associated risk factors or protective factors in human, environment and animal interface.

Indexed as

MilkMycobacterium bovisMycobacterium tuberculosisTuberculosis, BovineAnimalsBangladeshCattleEnzyme-Linked Immunosorbent AssayFemalePolymerase Chain ReactionPrevalenceRisk Factors

Identifiers

PMID41910171
PMCPMC13584884

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.