Evidence map›Paper›PMID 41909468›Full record

ArticleMolecular therapy. Nucleic acids2026

Potential effects of endogenous RNA/DNA hybrids on CRISPR-Cas9-mediated homology-directed repair.

Francesco Puzzo, Batuhan Bayram, Claudia Macaubas, Angela Lin, Hagoon Jang, Feijie Zhang, Elizabeth Mellins, Mark A Kay

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Molecular therapy. Nucleic acids, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Francesco PuzzoDepartment of Genetics, Stanford University, Stanford, CA 94305, USA.
Batuhan BayramDepartment of Pediatrics, Stanford University, Stanford, CA 94305, USA.
Claudia MacaubasDepartment of Pediatrics, Stanford University, Stanford, CA 94305, USA.
Angela LinDepartment of Genetics, Stanford University, Stanford, CA 94305, USA.
Hagoon JangDepartment of Genetics, Stanford University, Stanford, CA 94305, USA.
Feijie ZhangDepartment of Genetics, Stanford University, Stanford, CA 94305, USA.
Elizabeth MellinsDepartment of Pediatrics, Stanford University, Stanford, CA 94305, USA.
Mark A KayDepartment of Genetics, Stanford University, Stanford, CA 94305, USA.

Funding

BINDING OF MTP TO APOB DURING LIPOPROTEIN ASSEMBLYR01HL064272 · NHLBI · SUNY DOWNSTATE MEDICAL CENTER · PI HUSSAIN, M MAHMOOD · 1999 to 2006
$1.8M
NHLBI NIH HHS R01 HL064272
6 · The paper itself

Abstract

Recombinant adeno-associated virus (rAAV) vectors and CRISPR-Cas9 are widely used in gene therapy. However, how endogenous DNA secondary structures may potentially affect genome editing outcomes is not fully understood. RNA/DNA hybrids (R-loops), which form mostly during transcription, are dynamically regulated in cells and have been implicated in influencing DNA repair pathway choice. Here, we investigated whether genomic R-loops are associated with differences in Cas9-mediated genome editing outcomes

Indexed as

AAVCRISPR-Cas9DNA damage responseDNA repairgenome editinghomology-directed repairMT: RNA-DNA hybridsR-loops

Identifiers

PMID41909468
PMCPMC13022658

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.