Evidence map›Paper›PMID 41909173›Full record

ArticleNature reviews. Methods primers2025

Ptychography at all wavelengths.

Ruihai Wang, Qianhao Zhao, Lars Loetgering, Frederick Allars, Zhixuan Hong, Timothy J Pennycook, Roarke Horstmeyer, John Rodenburg, Andrew Maiden, Guoan Zheng

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Nature reviews. Methods primers, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Ruihai WangDepartment of Biomedical Engineering, University of Connecticut, Storrs, CT, USA.
Qianhao ZhaoDepartment of Biomedical Engineering, University of Connecticut, Storrs, CT, USA.
Lars LoetgeringZEISS Research Microscopy Solutions, Jena, Thuringia, Germany.
Frederick AllarsDiamond Light Source, Harwell, Oxfordshire, UK.
Zhixuan HongDepartment of Biomedical Engineering, University of Connecticut, Storrs, CT, USA.
Timothy J PennycookElectron Microscopy for Materials Science, University of Antwerp, Antwerpen, Belgium.
Roarke HorstmeyerDepartment of Biomedical Engineering, Duke University, Durham, NC, USA.
John RodenburgDepartment of Electronic and Electrical Engineering, University of Sheffield, Sheffield, UK.
Andrew MaidenDiamond Light Source, Harwell, Oxfordshire, UK.
Guoan ZhengDepartment of Biomedical Engineering, University of Connecticut, Storrs, CT, USA.

Funding

Label-free digital cytopathology using deep-ultraviolet coded ptychography with intrinsic molecular contrastR01EB034744 · NIBIB · UNIVERSITY OF CONNECTICUT STORRS · PI Guoan Zheng · 2023 to 2026
$1.4M
NIBIB NIH HHS R01 EB034744
6 · The paper itself

Abstract

Ptychography is a computational imaging technique that operates across multiple wavelength regimes, from electron (picometres) to X-ray (~0.1 nm), extreme ultraviolet (~10 nm), and visible light (micrometres). By reconstructing both amplitude and phase from diffraction patterns, ptychography enables high-resolution, quantitative imaging without conventional limitations imposed by lens-based optics. Ptychography has enabled advances across a range of scales: achieving record-breaking atomic resolution with electron microscopy, becoming an indispensable tool at X-ray synchrotron facilities worldwide, and overcoming the trade-offs between resolution and field-of-view in optical imaging. This Primer provides a unified treatment of ptychography across these wavelength regimes. First, we discuss theoretical foundations, reconstruction algorithms, experimental considerations and wavelength-specific challenges. We then give examples of raw and processed data from various configurations and wavelengths. Next, we highlight key applications of ptychography in life sciences, materials science and industry. We also discuss data standards, open-source software implementations, and best practices for ensuring reproducibility across different wavelength regimes. Finally, we consider limitations and future opportunities for ptychography. Together with accompanying datasets and code implementations, this Primer aims to serve newcomers and experienced practitioners in the field, facilitating broader adoption of ptychography across different disciplines.

Identifiers

PMID41909173
PMCPMC13024499

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.