Integrated metagenomic sequencing and phage display-based immunoprecipitation sequencing for presumptive viral infection: a case report.

Jessica G Shantha, Cindi Chen, Armin Hinterwirth, John A Gonzales, Nisha R Acharya, Thomas M Lietman, Thuy Doan

Abstract readCase Reports

In one paragraph

Article in American journal of ophthalmology case reports, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0cells of the map it votes in

0citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Jessica G ShanthaFrancis I. Proctor Foundation, University of California, San Francisco, CA, USA.

Cindi ChenFrancis I. Proctor Foundation, University of California, San Francisco, CA, USA.

Armin HinterwirthFrancis I. Proctor Foundation, University of California, San Francisco, CA, USA.

John A GonzalesFrancis I. Proctor Foundation, University of California, San Francisco, CA, USA.

Nisha R AcharyaFrancis I. Proctor Foundation, University of California, San Francisco, CA, USA.

Thomas M LietmanFrancis I. Proctor Foundation, University of California, San Francisco, CA, USA.

Thuy DoanFrancis I. Proctor Foundation, University of California, San Francisco, CA, USA.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Purpose: To report a case of uveitis in which unbiased metagenomic sequencing (MDS) and phage immunoprecipitation sequencing (PhIP-Seq) were performed on intraocular fluid. Observations: A female patient with a past medical history of human immunodeficiency virus (HIV-1) who presented with chronic active anterior and intermediate uveitis with cystoid macular edema in the left eye. She had a previous ocular history of viral retinitis presumed to be secondary to cytomegalovirus (CMV). An anterior chamber tap was performed for viral polymerase chain reactions (PCRs), MDS, and pathogen PhIP-Seq. PCR testing and MDS were negative for pathogen genetic materials. PhIP-Seq detected antibody enrichment of CMV, as well as HIV-1 andSARS-CoV-2. Conclusions: The combination of MDS and PhIP-Seq has the potential to provide additional insights into the pathogenesis and the ocular microenvironment of uveitis patients.

Indexed as

CytomegalovirusImmune reconstitution uveitis (IRU)Metagenomic sequencing (MDS)Phage Immunoprecipitation Sequencing (PhIP-Seq)UveitisViruses

Identifiers

PMID41908297

DOI10.1016/j.ajoc.2026.102561

PMCPMC13022695

What OpenQuestion holds

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LicenceCC BY-NC-ND

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.