Evidence map›Paper›PMID 41905069›Full record

ArticlePoultry science2026

Effects of dietary supplementation with polyphenolic extracts from Lasia spinosa Thw. roots on reproductive performance in Guangxi Ma Roosters.

Lang Zhang, Zhenlin Chen, Ying Zhang, Maosen Yang, Liping Pu, Haichuan Tan, Huiyan Xu, Mingxia Ran, Xingting Liu, Yangqing Lu

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Poultry science, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0citing papers in PubMed
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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Lang ZhangGuangxi Key Laboratory of Animal Breeding, Disease Control and Prevention, College of Animal Science and Technology, Guangxi University, Nanning, China.
Zhenlin ChenGuangxi Key Laboratory of Animal Breeding, Disease Control and Prevention, College of Animal Science and Technology, Guangxi University, Nanning, China.
Ying ZhangGuangxi Key Laboratory of Animal Breeding, Disease Control and Prevention, College of Animal Science and Technology, Guangxi University, Nanning, China.
Maosen YangGuangxi Key Laboratory of Animal Breeding, Disease Control and Prevention, College of Animal Science and Technology, Guangxi University, Nanning, China.
Liping PuGuangxi Key Laboratory of Animal Breeding, Disease Control and Prevention, College of Animal Science and Technology, Guangxi University, Nanning, China.
Haichuan TanGuangxi Zhuang Autonomous Region Wuxuan County Animal Disease Prevention and Control Center, Laibin 54590, Guangxi, China.
Huiyan XuGuangxi Key Laboratory of Animal Breeding, Disease Control and Prevention, College of Animal Science and Technology, Guangxi University, Nanning, China.
Mingxia RanGuangxi Key Laboratory of Animal Breeding, Disease Control and Prevention, College of Animal Science and Technology, Guangxi University, Nanning, China.
Xingting LiuGuangxi Key Laboratory of Animal Breeding, Disease Control and Prevention, College of Animal Science and Technology, Guangxi University, Nanning, China. Electronic address: xtliu@gxu.edu.cn.
Yangqing LuGuangxi Key Laboratory of Animal Breeding, Disease Control and Prevention, College of Animal Science and Technology, Guangxi University, Nanning, China. Electronic address: lyq@gxu.edu.cn.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Reproductive performance in breeder roosters is a critical determinant of egg fertilization rates and the long-term sustainability of poultry breeding enterprises. Lasia spinosa Thw. (LST) possesses bioactive potential to modulate hormonal profiles and spermatogenic functions in male animals. However, research on the functional roles of its specific phytochemical constituents remains limited. This study aimed to evaluate the effects of dietary supplementation with polyphenols extracts from Lasia spinosa Thw. (LST_PP) on rooster reproductive performance and to elucidate the underlying regulatory mechanisms. Forty roosters were randomly assigned to four groups: a control group receiving a basal diet and three treatment groups receiving the same basal diet supplemented with LST_PP at 500, 1,000, or 2,000 mg/kg, respectively, for 60 consecutive days. Results showed that dietary supplementation with LST_PP had no significant effect on body weight, feed intake or general health status. However, the group supplemented with 1,000 mg/kg LST_PP exhibited a significant increase in comb height compared to the control group (P < 0.05). Semen quality analysis revealed that LST_PP supplementation improved semen parameters in roosters, including semen volume, sperm density, and sperm motility, with the 1,000 mg/kg group exhibiting significantly higher values than the control group (P < 0.05). Additionally, testicular weight and the testicular index in the 1,000 mg/kg group were significantly higher compared to the control (P < 0.05). Hormonal assays revealed significantly elevated serum testosterone and luteinizing hormone (LH) levels in the 1,000 mg/kg LST_PP group, whereas testicular testosterone content was significantly increased in both the 1,000 mg/kg and 2,000 mg/kg groups (P < 0.05). Histological evaluation demonstrated that LST_PP significantly increased the diameter of seminiferous tubules and the height of spermatogenic epithelium (P < 0.05). Furthermore, dietary supplementation with LST_PP also significantly enhanced testicular antioxidant capacity. Network pharmacology analysis identified steroid hormone biosynthesis and PI3K-Akt signaling pathways as the key mediators regulating the pro-reproductive effects of LST_PP's, with quercetin and kaempferol proposed as the core bioactive components. In conclusion, dietary LST_PP supplementation effectively enhances rooster reproductive performance, and the optimal dosage was found to be 1,000 mg/kg.

Indexed as

ApocynaceaeChickensPlant ExtractsPolyphenolsReproductionAnimal FeedAnimalsDietDietary SupplementsDose-Response Relationship, DrugMalePlant RootsRandom AllocationSemen AnalysisTestisPlant ExtractsPolyphenolsPolyphenolic extracts from Lasia spinosa Thw. rootsReproductive performanceRoosterSemen qualityTesticular development

Identifiers

PMID41905069
PMCPMC13054074

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.