Evidence map›Paper›PMID 41904955›Full record

ArticleSTAR protocols2026

Protocol for characterization of spatiotemporal network dynamics in cortical and hippocampal assembloids.

Asia R Guzman, Colin M McCrimmon, Daniel Toker, Bennett G Novitch, Momoko Watanabe, Ranmal A Samarasinghe

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in STAR protocols, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Asia R GuzmanDepartment of Neurology, University of California, Los Angeles, David Geffen School of Medicine, Los Angeles, CA 90095, USA.
Colin M McCrimmonDepartment of Neurology, University of California, Los Angeles, David Geffen School of Medicine, Los Angeles, CA 90095, USA.
Daniel TokerDepartment of Neurology, University of California, Los Angeles, David Geffen School of Medicine, Los Angeles, CA 90095, USA.
Bennett G NovitchDepartment of Neurobiology, University of California, Los Angeles, Los Angeles, CA 90095, USA; Eli and Edythe Broad Center for Regenerative Medicine and Stem Cell Research, University of California, Los Angeles, Los Angeles, CA 90095, USA; Intellectual Development and Disabilities Research Center, University of California, Los Angeles, Los Angeles, CA 90095, USA.
Momoko WatanabeDepartment of Anatomy and Neurobiology, School of Medicine, University of California, Irvine, Irvine, CA, USA; Sue & Bill Gross Stem Cell Research Center, School of Medicine, University of California, Irvine, Irvine, CA, USA. Electronic address: momokow@uci.edu.
Ranmal A SamarasingheDepartment of Neurology, University of California, Los Angeles, David Geffen School of Medicine, Los Angeles, CA 90095, USA; Eli and Edythe Broad Center for Regenerative Medicine and Stem Cell Research, University of California, Los Angeles, Los Angeles, CA 90095, USA; Intellectual Development and Disabilities Research Center, University of California, Los Angeles, Los Angeles, CA 90095, USA. Electronic address: rsamarasinghe@mednet.ucla.edu.

Funding

Integrative Modeling of HIV-Associated Neurocognitive Disorder in Human Brain OrganoidsR01DA051897 · NIDA · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI FREGOSO, OLIVER I, NOVITCH, BENNETT G · 2020 to 2024
$3.1M
Utilizing Human Brain Organoids to Model the Differential Effects of SCN8A Mutation on Cortex and HippocampusK08NS119747 · NINDS · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI SAMARASINGHE, RANMAL A · 2021 to 2025
$1.1M
NIDA NIH HHS R01 DA051897NINDS NIH HHS K08 NS119747
6 · The paper itself

Abstract

This protocol generates neural assembloids from human induced pluripotent stem cells (hiPSCs) to model human brain circuitry. It details the differentiation of excitatory-predominant hippocampal (Hc) and cortical (Cx) organoids and their fusion with inhibitory interneuron-predominant ganglionic eminence (GE) organoids. We then detail procedures for maintaining assembloids to promote interneuron migration and network integration, followed by functional assessment using two-photon calcium imaging to measure neuronal activity. For complete details on the use and execution of this protocol, please refer to McCrimmon et al.

Indexed as

Cell Culture TechniquesCerebral CortexHippocampusNerve NetOrganoidsCell DifferentiationHumansInduced Pluripotent Stem CellsInterneuronsNeuroscienceOrganoidsStem Cells

Identifiers

PMID41904955
PMCPMC13054417

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.