Evidence map›Paper›PMID 41903458›Full record

ArticlePoultry science2026

Dose-dependent effects of Nymphaea lotus extract in semen extenders on sperm quality and fertility in Thai native roosters during cooled and cryopreserved storage.

Thirawat Koedkanmark, Khomsan Buathalad, Wuttigrai Boonkum, Yoswaris Semaming, Vibuntita Chankitisakul

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In one paragraph

Article in Poultry science, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Thirawat KoedkanmarkDepartment of Animal Science, Faculty of Agriculture, Khon Kaen University, Khon Kaen 40002, Thailand.
Khomsan BuathaladDepartment of Animal Science, Faculty of Agriculture, Khon Kaen University, Khon Kaen 40002, Thailand.
Wuttigrai BoonkumDepartment of Animal Science, Faculty of Agriculture, Khon Kaen University, Khon Kaen 40002, Thailand; Network Center for Animal Breeding and Omics Research, Khon Kaen University, Khon Kaen 40002, Thailand.
Yoswaris SemamingProgram in Veterinary Technology, Faculty of Technology, Udon Thani Rajabhat University, Udon Thani, Thailand.
Vibuntita ChankitisakulDepartment of Animal Science, Faculty of Agriculture, Khon Kaen University, Khon Kaen 40002, Thailand; Network Center for Animal Breeding and Omics Research, Khon Kaen University, Khon Kaen 40002, Thailand. Electronic address: vibuch@kku.ac.th.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

This study evaluated the dose-dependent effects of Nymphaea lotus extract supplementation in semen extenders on sperm quality and fertility in Thai native roosters under cooled and cryopreserved storage conditions. Phytochemical analysis revealed that the ethanolic extract contained high levels of total phenolics (596 mg/g DW) and proanthocyanidins (295 mg/g DW), along with flavonoids and tannins. In vitro antioxidant assays demonstrated strong radical-scavenging and ferric-reducing activities, with IC₅₀ values comparable to standard antioxidants, and significant protection against AAPH-induced hemolysis. For cooled semen stored at 5°C, supplementation with 0.10 mg/mL N. lotus significantly improved sperm motility and viability and reduced malondialdehyde levels for up to 48 h (P < 0.05), with fertility significantly enhanced at 24 h of storage. Although fertility at 48 h did not differ significantly from the control group, absolute fertility values remained high. In cryopreserved semen, supplementation with 0.025 mg/mL resulted in the highest post-thaw sperm motility, viability, and fertility (P < 0.05). These findings demonstrate that N. lotus extract is an effective natural antioxidant for improving rooster semen quality and fertilizing efficiency, with optimal supplementation levels dependent on the storage method.

Indexed as

ChickensCryopreservationNelumboPlant ExtractsSemen PreservationSpermatozoaAnimalsAntioxidantsCryoprotective AgentsDose-Response Relationship, DrugFertilityMaleSemenSemen AnalysisSperm MotilityThailandAntioxidantsCryoprotective AgentsPlant ExtractsAntioxidantArtificial inseminationAvian reproductionOxidative stressSemen preservation

Identifiers

PMID41903458
PMCPMC13054019

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.